生物技术通报2025，Vol.41Issue(11)：75-88,14.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0153
5-氨基乙酰丙酸(5-ALA)的生物合成研究进展
Advances in the Biosynthesis of 5-aminolevulinic Acid(5-ALA)
摘要
Abstract
5-aminolevulinic acid(5-ALA)is a non-protein amino acid naturally occurring in living organisms,and has been widely used in the fields of medicine,health care,agriculture and animal additives,etc.5-ALA is mainly synthesized by chemical and biological methods,but the complexity of the chemical synthesis process and cost problems have limited its large-scale development,and biosynthesis method has highlighted its great potential for industrial development due to its advantages of environmental protection and high efficiency.The biosynthesis method,with its advantages of environmental protection and high efficiency,has shown its great potential for industrial development.In recent years,the biosynthesis of 5-ALA has gradually become a research hotspot due to the rapid development of interdisciplinary disciplines such as synthetic biology and metabolic engineering.In this paper,we reviewed the development of 5-ALA biosynthesis in the past decades,summarized the progress of various metabolic engineering strategies based on the C4 and C5 pathways,cofactor regeneration pathway,tricarboxylic acid cycle and other pathways for the construction of high-efficiency cell factories,and highlighted the application and significance of the dynamic regulation based on biosensors and the establishment of high-throughput screening methods in the metabolic engineering modification of the bacterial strains.We also put forward several strategies and prospects for further improving the synthetic yield of 5-ALA and breaking the bottleneck of its industrial application,with a view to providing certain references and research bases for the efficient synthesis and industrial manufacturing of 5-ALA.关键词
5-氨基乙酰丙酸/生物合成/代谢工程/动态调控/生物传感器Key words
5-aminolevulinic acid/biosynthesis/metabolic engineering/dynamic regulation/biosensors引用本文复制引用
吕欢欢,张高阳,王赛笛,孙忠科,李成伟,罗德平..5-氨基乙酰丙酸(5-ALA)的生物合成研究进展[J].生物技术通报,2025,41(11):75-88,14.基金项目
河南省重大科技专项(231100110300),河南工业大学高层次人才基金项目(2021BS017) （231100110300）
