生物技术通报2025，Vol.41Issue(11)：89-99,11.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0146
CRISPR/Cas9技术在益生菌编辑中的应用与进展
Application and Progress of CRISPR/Cas9 Technology in Probiotic Editing
摘要
Abstract
Gene editing is an advanced biotechnology that allows for the efficient and precise modification of the DNA sequence of an organism's genome,enabling the regulation gene functions or the improvement of traits.In recent years,the revolution of gene editing technology has opened up new avenues for the optimization of probiotic functions.The engineering of prob through gene editing has been widely applied in agriculture,medicine,production,and scientific research.Among them,CRISPR/Cas9,as a part of the bacterial adaptive immune system,has a higher editing efficiency and lower cost of use compared with traditional zine finger endonuclease and transcription activator-like effector nuclease technology.CRISPR/Cas9 has rapidly become a revolutionary gene editing tool in the field of life sciences.At present,the probiotic industry is developing rapidly,and CRISPR/Cas9 technology has successfully achieved precise gene modification in bacteria,cells,animals and various plants.With the continuous deepening of research,CRISPR/Cas9 technology provides more efficient and precise modification strategies for probiotic gene editing by optimizing and sgRNA,selecting new nucleases,using dual CRISPR cutting systems combining base editing technology and other ways.This article first gives a brief introduction to CRISPR/Cas9 technology,elucidates its structural composition and action mechanism,then explores the necessity and potential value of editing probiotics through CRISPR/Cas9 technology,and combines specific examples to show the current practical application status of this technology in the field of probiotics in detail.Then,the article p lists the issues of high off-target rate,chromosomal abnormalities,and edited cytotoxicity.Finally,the article prospects the application trends of CRISPR/Cas9 technology,and points out that its healthy and orderly development can not be without the supervision of policies and laws,aiming to provide references for the application of CRISPR/Cas9 technology in probiotic editing.关键词
CRISPR/Cas9/基因编辑/益生菌/菌株功能定制/基因功能分析/疾病治疗Key words
CRISPR/Cas9/gene editing/probiotics/strain function customization/gene function analysis/disease treatment引用本文复制引用
刘梓琦,钟沛,李琴,郭成,张艳梅,张乃锋,屠焰,刁其玉,毕研亮..CRISPR/Cas9技术在益生菌编辑中的应用与进展[J].生物技术通报,2025,41(11):89-99,11.基金项目
国家重点研发计划项目(2022YFA1304200),国家自然科学基金项目(32222081,32372912) （2022YFA1304200）
