陕西医学杂志2025，Vol.54Issue(12)：1587-1595,9.DOI:10.3969/j.issn.1000-7377.2025.12.001
基于网络药理学和体外实验模型探讨淫羊藿素对肝细胞癌的抑制作用及潜在机制
Inhibitory effect and potential mechanism of icaritin on hepatocellular carcinoma were explored based on network pharmacology and in vitro experimental models
摘要
Abstract
Objective:To explore the inhibitory effect of icaritin(ICA)on hepatocellular carcinoma(HCC)and its potential mechanism based on network pharmacology and in vitro experiments.Methods:ICA and HCC-related targets were identified via literature review and drug and disease-related databases.Venn diagrams facilitated the vi-sualization and analysis of intersecting genes between the two.A protein-protein interaction network was constructed to identify core targets and analyze gene enrichment and biological processes.Based on network pharmacology analy-sis,the CCK-8 assay was employed to evaluate the effect of ICA on HepG2 cell proliferation.The scratch healing as-say assessed ICA's impact on cell migration capacity.Flow cytometry was utilized to examine the effect of ICA on cell apoptosis.The expression changes of apoptosis-related proteins and proteins related to PI3 K/AKT pathway were de-tected by Western blot.Results:A comprehensive screening identified 113 targets associated with ICA and 8547 tar-gets related to HCC,resulting in the identification of 77 potential targets for ICA's action on HCC through cross-ref-erencing.Protein-protein interaction(PPI)network analysis revealed 10 core genes.Enrichment analysis indicated that ICA exerts its anti-HCC effects by modulating multiple signaling pathways,with the PI3 K/AKT pathway poten-tially serving as the primary regulatory pathway.Results from CCK-8 and scratch assays demonstrated that ICA in-hibited the proliferation and migration of HepG2 cells in a concentration-dependent manner(all P<0.05).Flow cy-tometry analysis revealed that different concentrations of ICA significantly induced apoptosis in HepG2 cells(all P<0.05).Western blot analysis showed that ICA treatment significantly decreased the protein expression levels of p-PI3 K,p-AKT,Bcl-2,and Pro-caspase-3 in HepG2 cells,while significantly increasing the expression of Bax and Cleaved-caspase-3(all P<0.05);upon combination with the PI3 K activator 740Y-P,the ICA-740Y-P treatment group demonstrated a significant reversal of the decreased levels of p-PI3 K,p-AKT,and apoptosis-related proteins,in comparison to treatment with ICA alone(all P<0.05).Conclusion:ICA can effectively inhibit the proliferation and migration of HCC cells and induce cell apoptosis.This may be related to its ability to inhibit the activation of the PI3 K/AKT pathway.关键词
肝细胞癌/淫羊藿素/网络药理学/磷脂酰肌醇3-激酶/蛋白激酶B/信号通路Key words
Hepatocellular carcinoma/Icaritin/Network pharmacology/Phosphatidylinositol 3-kinase/Protein kinase B/Signaling pathway分类
医药卫生引用本文复制引用
李禄阳,柳成刚..基于网络药理学和体外实验模型探讨淫羊藿素对肝细胞癌的抑制作用及潜在机制[J].陕西医学杂志,2025,54(12):1587-1595,9.基金项目
国家中医药管理局龙江医学流派传承工作室建设项目(LPGZS2012-14) （LPGZS2012-14）
