陕西医学杂志2025，Vol.54Issue(12)：1608-1615,8.DOI:10.3969/j.issn.1000-7377.2025.12.004
杜仲水提取物通过PI3K/AKT途径调节铁代谢改善糖尿病性骨质疏松症实验研究
Eucommia ulmoides water extract regulates iron metabolism through PI3 K/AKT pathway to improve diabetic osteoporosis
摘要
Abstract
Objective:To investigate the effect of Eucommia ulmoides(EUL)water extract on the improvement of diabetic osteoporosis(DOP)through regulating iron metabolism via the phosphatidylinositol 3-kinase(PI3 K)/protein kinase B(AKT)pathway.Methods:Sixty SD rats were randomly divided into the control group,the DOP group and the EUL group,with 20 rats in each group.The DOP rat model was established by using a high-fat diet combined with streptozotocin injection.The EUL group rats were orally administered 2.5 g/(kg d)of EUL water extract,while the control group and the DOP group were orally administered the same amount of distilled water,for a duration of 12 weeks.Micro-CT was used to evaluate bone mineral density(BMD),trabecular number(Tb.N)and trabecular volume/total volume(BV/TV).Serum iron ions and malondialdehyde(MDA)levels were detected,and the expression of proteins related to the PI3 K/AKT signaling pathway was analyzed by Western blot.The MC3T3-E1 osteoblast cell model was used to analyze the effects of EUL water extract on cell proliferation,osteogenic differ-entiation and iron metabolism.Results:Compared with the control group,the BMD,Tb.N and BV/TV in the DOP group were significantly decreased,while those in the EUL group were significantly increased compared with the DOP group(all P<0.05).EUL treatment significantly reduced the serum iron ions and MDA levels in DOP rats,and simultaneously increased the expression of p-PI3 K and p-AKT proteins in bone tissue(all P<0.05).In MC3T3-E1 cells,EUL significantly enhanced the cell proliferation ability,ALP activity and calcium deposition under high glu-cose conditions,and improved the iron metabolism disorder of the cells.The improvement effect of EUL was inhibi-ted after adding the PI3 K inhibitor LY294002.Conclusion:The EUL water extract can effectively alleviate iron me-tabolism disorders and osteogenic dysfunction in the DOP model by activating the PI3 K/AKT signaling pathway.关键词
糖尿病性骨质疏松症/杜仲水提取物/磷脂酰肌醇3-激酶/蛋白激酶B/信号通路/铁代谢/骨再生Key words
Diabetic osteoporosis/Eucommia ulmoides water extract/Phosphatidylinositol 3-kinase/Protein kinase B/Signaling pathway/Iron metabolism/Bone regeneration分类
医药卫生引用本文复制引用
罗红清,邓洁,徐丽,卢靖,李洪辉,邝涛,刘采群..杜仲水提取物通过PI3K/AKT途径调节铁代谢改善糖尿病性骨质疏松症实验研究[J].陕西医学杂志,2025,54(12):1608-1615,8.基金项目
湖南省中医药科研课题(B2024042) （B2024042）
