陕西医学杂志2025，Vol.54Issue(12)：1616-1621,6.DOI:10.3969/j.issn.1000-7377.2025.12.005
基于SCF/C-kit信号通路探讨川陈皮素对慢性传输型便秘大鼠肠道蠕动的改善作用实验研究
Improvement effect of nobiletin on intestinal peristalsis in rats with chronic transit constipation based on SCF/C-kit signaling pathway
摘要
Abstract
Objective:To investigate the improvement effect of nobiletin on intestinal peristalsis in rats with slow transit constipation(STC)based on the stem cell factor(SCF)/tyrosine kinase receptor(C-kit)signaling path-way.Methods:Forty SPF-grade SD rats were selected as experimental animals,and 10 rats were randomly selected as blank group,and the rest were established as chronic transmission constipation model,and 27 rats with successful modeling were divided into the model group,nobiletin group and nobiletin+ISCK03 group,with 9 rats in each group.Comparison of serum indexes,pathomorphologic changes,small intestinal propulsion rate,fecal water content,fecal excretion,gastric emptying rate,charcoal ink propulsion rate,and the mRNA and protein expressions of SCF and C-kit were made in each group.Results:Compared with the blank group,gastric emptying rate,small intestinal pro-pulsion rate,fecal water content,fecal excretion,charcoal ink propulsion rate,charcoal ink propulsion length,SP and 5-HT levels,SCF and C-kit mRNA and protein decreased,and the levels of NOS,VIP,IL-6,IL-1β,and TNF-αin-creased in the model group,nobiletin group,and nobiletin+ISCK03 group(all P<0.05).Compared with the model group,the small intestinal propulsion rate,fecal water content,fecal excretion,gastric emptying rate,charcoal ink pro-pulsion rate,charcoal ink propulsion length,SP and 5-HT levels,SCF and C-kit mRNA and protein were elevated,and the levels of IL-6,IL-1β,TNF-α,NOS,and VIP were decreased in the nobiletin group(all P<0.05).Compared with the nobiletin group,gastric emptying rate,small intestinal propulsion rate,charcoal ink propulsion rate,charcoal ink propulsion length,fecal water content,fecal excretion,SP and 5-HT levels,SCF and C-kit mRNA and protein were decreased,and the levels of NOS,VIP,IL-6,IL-1β,and TNF-αwere elevated in the nobiletin+ISCK03 group(all P<0.05).Conclusion:Nobiletin may regulate the intestinal peristalsis of STC rats by modulating the SCF/C-kit path-way,thereby alleviating constipation symptoms.关键词
慢性传输型便秘/川陈皮素/干细胞因子/酪氨酸激酶受体信号通路/肠道蠕动/大鼠Key words
Slow transit constipation/Nobiletin/SCF/C-kit signaling pathway/Intestinal peristalsis/Rats分类
医药卫生引用本文复制引用
夏婧,陈伟特..基于SCF/C-kit信号通路探讨川陈皮素对慢性传输型便秘大鼠肠道蠕动的改善作用实验研究[J].陕西医学杂志,2025,54(12):1616-1621,6.基金项目
福建医学科技青年培育项目(22FBQN2022114) （22FBQN2022114）
