实用医学杂志2025，Vol.41Issue(23)：3638-3644,7.DOI:10.3969/j.issn.1006-5725.2025.23.002
抗阻运动调节Piezo1对老年小鼠骨骼肌成肌细胞的影响
The effect of resistance exercise regulation of Piezo1 on myoblasts in skeletal muscles in elderly mice
摘要
Abstract
Objective To investigate the regulatory effects of resistance exercise on Piezo1 in myoblasts within skeletal muscles of aged mice.Methods In animal experiments,thirty 20-month-old male C57BL/6 mice were randomly assigned to one of three groups:an aging control group,an aging exercise group,and an aging exercise inhibitor group.Additionally,ten 3-month-old young mice served as a young control group.The aging exercise group underwent 8 weeks of resistance exercise training,whereas the aging exercise inhibitor group received both 8 weeks of resistance exercise and intraperitoneal injections of GsMTx4.Exercise capacity was evaluated using grip strength tests and rotarod performance assays.Skeletal muscle cross-sectional area was assessed via hematoxylin-eosin staining,fibrosis was examined using Sirius red staining,and the proportion of p16-positive cells was determined by immunohistochemistry.In cellular experiments,senescence in myoblasts was induced with D-galactose(D-gal),followed by activation of Piezo1 using Yoda1.The percentage of senescent cells was measured by SA-β-Gal staining,while protein expression levels of senescence markers(p16 and p21)and atrophy markers(Atrogin-1 and MuRF1)were quantified by Western blot analysis.Cell immunofluorescence staining was employed to assess the average fluorescence intensity of p21.Results(1)Compared with the aging control group,the aging+exercise group exhibited significant improvements in exercise capacity,quadriceps femoris mass index,skeletal muscle cross-sectional area,reduction in skeletal muscle fibrosis,and a lower p16-positive cell percentage(P<0.05);(2)These beneficial effects of exercise on aged skeletal muscle were attenuated following treatment with a Piezo1 inhibitor(P<0.05);(3)In vitro experiments demonstrated that activation of Piezo1 reduced the SA-β-Gal positive cell rate in myoblasts,downregulated the expression of senescence markers(p16 and p21)and atrophy-related markers(Atrogin-1 and MuRF1),and decreased the average fluorescence intensity of p21(P<0.05).Conclusion Resistance exercise suppresses myocyte aging through activation of Piezo1,thereby ameliorating age-related muscle atrophy in the elderly.关键词
老年性肌萎缩/抗阻运动/Piezo1/衰老/骨骼肌Key words
annual muscular atrophy/resistance exercise/Piezo1/aging/skeletal muscles分类
医药卫生引用本文复制引用
林榕,刘刚..抗阻运动调节Piezo1对老年小鼠骨骼肌成肌细胞的影响[J].实用医学杂志,2025,41(23):3638-3644,7.基金项目
国家自然科学基金项目(编号:82274619,82302846) （编号:82274619,82302846）
广东省自然科学基金项目(编号:2024A1515012122) （编号:2024A1515012122）
