西北农业学报2025，Vol.34Issue(11)：1983-1993,11.DOI:10.7606/j.issn.1004-1389.2025.11.002
红嘴鸥与3种家禽IFN-α基因比较研究
Comparative Study on IFN-α Genes between Larus ridibundus and Three Domestic Poultry Species
摘要
Abstract
This study cloned the interferon-α(IFN-α)gene from Larus ridibundus and conducted a comparative analysis with IFN-α gene sequences from three domestic poultry species-chicken,duck,and goose to support future studies on the expression and functional characterization of the IFN-α pro-tein of L.ridibundusn.The full-length IFN-α gene of L.ridibundus,579 bp long,was successfully obtained using PCR amplification and sequencing.Sequence comparison revealed differences in se-quence length and similarity between L.ridibundus and the three poultry species,with L.ridibundus showing a closest phylogenetic relationship to Rissa tridactyla.Protein sequence analysis indicated that all four IFN-α proteins are hydrophobic,lack transmembrane domains,and possess signal pep-tides,glycosylation sites,and phosphorylation sites,although these functional elements are located at different amino acid positions.Subcellular localization predictions showed that all four proteins are primarily located in the nucleus,albeit with varying distribution levels.Furthermore,secondary and tertiary structure predictions suggested that α-helices are the dominant structural elements across all four IFN-α proteins,although the specific positions of these helices vary.These findings provide foun-dational data for the recombinant expression and functional investigation of L.ridibundus IFN-α pro-tein in future antiviral studies.关键词
红嘴鸥/干扰素α(IFN-α)/基因比对分析Key words
Larus ridibundus/Interferon-α(IFN-α)/Gene alignment analysis引用本文复制引用
马俊蕊,张雪,王一涵,张宏莉,段纲,项勋,常华..红嘴鸥与3种家禽IFN-α基因比较研究[J].西北农业学报,2025,34(11):1983-1993,11.基金项目
云南省重大科技专项计划"抗畜禽腹泻兽药创制"项目(202302AA310020) （202302AA310020）
云南省重大科技专项计划"云南高原猪鸡饲料高效生产与利用关键技术研发与应用"项目(202202AE090024) （202202AE090024）
云南农业大学第四批校级一流本科课程"兽医分子生物学技术"(A1010103001). Special Program of Yunnan Provincial Major Science and Technology"Creation of Veterinary Drugs for Anti-Livestock and Poultry Diarrhea"(No.202302AA310020) （A1010103001）
Yunnan Provin-cial Major Science and Technology Special Program"R&D and Application of Key Technologies for Efficient Production and Utilization of Pig and Chicken Feed on Yunnan Plateau"(No.202202AE090024) （No.202202AE090024）
the Fourth Batch of First-class Undergraduate Course"Veterinary Molecular Biol-ogy Technology"of Yunnan Agricultural University(No.A1010103001). （No.A1010103001）
