西北农业学报2025，Vol.34Issue(11)：2055-2067,13.DOI:10.7606/j.issn.1004-1389.2025.11.009
基于转录组分析黑果越橘对高温胁迫的响应机制
Response Mechanism of Vaccinium myrtillus L.Stressed by High Temperature Based on Transcriptome Analysis
摘要
Abstract
Vaccinium myrtillus L.is a deciduous shrub growing at high altitude with high medicinal value.High temperature is one of the main factors affecting its introduction and cultivation.To explore the adaptability of Vaccinium myrtillus L.to high temperature during the introduction and cultivation process,and to investigate the effects of high temperature stress on differential genes and key meta-bolic pathway enzyme genes in metabolic pathways,transcriptome sequencing was performed at vari-ous time points under such stress conditions.The transcriptome of Vaccinium myrtillus L.after high-temperature treatment for 0,12 and 24 hours was determined using RNA-Seq technology,followed by a bioinformatics analysis.The results showed that a total of 89 204 Unigenes were obtained,with an average length of 1 152 bp and a N50 length of 1 894 bp.In 7 databases including GO and KEGG,a total of 66,140 Unigenes were annotated,representing 74.14％of the total.Among these,35 149 Unigenes were annotated in GO and 33 545 in KEGG.The GO analysis of 3 244 differentially ex-pressed genes indicated their primary involvement in cellular processes,metabolic processes,cells,membranes,and organelles.The emphasis of the KEGG enrichment analysis was on ribosomes,oxi-dative phosphorylation,protein processing in the endoplasmic reticulum,and glutathione metabo-lism.High temperature induced differential expression of genes associated with glutathione metabo-lism,flavonoid biosynthesis,and protein processing in the endoplasmic reticulum.Such stress also mitigated heat damage and influenced flavonoid accumulation.The qRT-PCR verification results aligned with those of RNA-Seq sequencing,confirming the reliability of the transcriptome data.Initial research has indicated that Vaccinium myrtillus L.can control key enzyme genes in glutathione me-tabolism and flavonoid biosynthesis when exposed to high temperature stress.These results provide new insights into the introduction and cultivation of Vaccinium myrtillus L.for high-temperature ad-aptation,help elucidate the mechanisms underlying its response to heat stress,and offer data support for future studies on functional genes involved in this process.关键词
黑果越橘/高温/转录组分析/差异表达基因Key words
Vaccinium myrtillus L./High temperature/Transcriptome analysis/Differentially ex-pressed genes引用本文复制引用
谷玉风,刘欣欣,杨延龙,陈勇,乔旭,马君,葛风伟,赖成霞..基于转录组分析黑果越橘对高温胁迫的响应机制[J].西北农业学报,2025,34(11):2055-2067,13.基金项目
新疆维吾尔自治区重点研发计划(2023B02023-1). Key R&D Program Project of Xinjiang Uygur Autonomous Region(No.2023B02023-1). （2023B02023-1）
