西南农业学报2025，Vol.38Issue(10)：2120-2128,9.DOI:10.16213/j.cnki.scjas.2025.10.008
柑橘BOR基因家族的全基因组鉴定及其表达分析
Genome-wide identification and expression analysis of BOR gene family in citrus
摘要
Abstract
[Objective]The paper aimed to identify and analyze the biological information of the BOR gene family in citrus,the gene expres-sion and functions under boron treatment,thus providing a theoretical basis for the analysis of the mechanism of efficient boron utilization in citrus and molecular breeding of boron-tolerant varieties.[Method]By integrating the genomic data of Citrus sinensis,C.grandis,C.clementi-na and using the hidden Markov model(PF00955)to screen members of the BOR family,the gene structure,conserved motif and phyloge-netic tree were analyzed by combining MEME and TB-tools.The promoter cis-acting elements were predicted based on PlantCARE.The tissue specific expression of BOR gene was further analyzed based on transcriptome data,and the expression patterns of CsBOR genes in roots,stems and leaves treated with different boron concentrations(0,200,400 μmol/L)were verified by qPCR.[Result]A total of 18 members of BOR were identified based on the genome of C.sinensis,C.grandis and C.clementina.They were divided into two subfamilies by cluster analysis.The gene structure and promoter elements showed that the gene structure of 18 BOR members in citrus were highly similar.In the promoter re-gion,10 hormone response elements and 6 abiotic response elements were detected.The transcriptome data of C.sinensis showed that Cs-BOR2,CsBOR4 and CsBOR5 were highly expressed in all tissues.qPCR results showed that CsBOR1 and CsBOR5 expression were signifi-cantly up-regulated in leaves,and CsBOR2 and CsBOR4 expression were significantly induced in roots under high boron concentration.[Con-clusion]The study systematically analyzes the characteristics of the citrus BOR gene family and the expression patterns of CsBOR genes under boron treatment,indicating that the C.sinensis BOR genes exhibit different expression patterns in different tissues treated with various concen-trations of boron.This may be related to the different demands of boric acid in different tissues of C.sinensis and the differentiation of gene functions.关键词
柑橘/基因家族/BOR/基因表达/硼Key words
Citrus/Gene family/BOR/Gene expression/Boron分类
农业科技引用本文复制引用
陈彤,杨金磊,赵玉双,吴海玲,卢嘉伟,朱倩,成锐,陈岳文..柑橘BOR基因家族的全基因组鉴定及其表达分析[J].西南农业学报,2025,38(10):2120-2128,9.基金项目
现代农业产业技术体系建设专项(CARS-26) （CARS-26）
湖南省自然科学基金青年项目(2024JJ6240) （2024JJ6240）
湖南省教育厅科学研究优秀青年项目(2B0191) （2B0191）
