新医学2025，Vol.56Issue(11)：1064-1072,9.DOI:10.12464/j.issn.0253-9802.2025-0173
芹菜素在MMTV-PyMT自发乳腺癌小鼠肿瘤生长和肺转移中的作用
Effect of apigenin on spontaneous breast cancer growth and pulmonary metastasis in MMTV-PyMT and its mechanism
摘要
Abstract
Objective To evaluate the effect of apigenin on breast cancer growth and lung metastasis in MMTV-PyMT.Methods MMTV-PyMT female mice aged 9-week were selected and divided into the apigenin treatment and control groups.In the apigenin treatment group(n=5),mice were treated with intraperitoneal injection of 5 mg/kg apigenin,while those in the control group(n=5)were given with intraperitoneal injection of DMSO at the equivalent dose for 4 weeks.Body weight and tumor volume of the mice were measured during the treatment.After 4 weeks,the tumors and lung tissues of the mice were isolated.Tumor weight was measured,and the number of nodules on the lung surface was counted.Immunohistochemical staining was used to detect the number of proliferating cells and microvessels in the tumor tissues.Results Compared with the control group,the tumor volume of the apigenin-treated mice was significantly smaller and tumor weight was significantly lighter than than those in the control group.Moreover,the number of metastatic nodules on the lung surface in the apigenin treatment group was significantly lower than that in the control group(all P＜0.05).The results of immunohistochemistry showed that the number of proliferating cells(Ki67)and microvessel density in the apigenin treatment group were significantly lower than those in the control group(both P＜0.05).Conclusion Apigenin significantly inhibits the growth and lung metastasis of breast cancer in MMTV-PyMT mice probably by inhibiting tumor cell proliferation and angiogenesis.关键词
芹菜素/多瘤病毒中间T抗原小鼠/乳腺癌/生长/转移/血管生成Key words
Apigenin/MMTV-PyMT/Breast cancer/Growth/Metastasis/Angiogenesis引用本文复制引用
占雅平,何亚军,王莹,徐能,亓翠玲..芹菜素在MMTV-PyMT自发乳腺癌小鼠肿瘤生长和肺转移中的作用[J].新医学,2025,56(11):1064-1072,9.基金项目
广东省自然科学基金项目(2023A1515010459) （2023A1515010459）
广东省中医药管理局科研项目(20251209) （20251209）
