医学新知2025，Vol.35Issue(11)：1279-1285,7.DOI:10.12173/j.issn.1004-5511.202505011
RhD初筛阴性献血者RhCE表型分布和D变异型分子遗传背景研究
The distribution of RhCE phenotypes and molecular genetic background of D variants in preliminary screening of RhD-negative blood donors
摘要
Abstract
Objective To explore the distribution of RhCE phenotypes and the molecular genetic background of D variants among preliminary screening RhD-negative blood donors.Methods Blood samples sent for testing to the Linyi Central Blood Station from July 2022 to June 2023 were collected.The Rh phenotype was detected using the test tube method,and RhD blood type confirmation was performed using a broad-spectrum anti-human globulin card(microcolumn method).For samples serologically confirmed as RhD variant,the RHD gene was tested using PCR-SSP.Sequencing analysis was performed on samples with positive amplification of all or part of the exons.Results A total of 564 cases were initially screened as RhD negative.The ABO blood type distribution was as follows:O type(32.98%),A type(27.48%),B type(26.06%),and AB type(13.48%).The confirmation results for RhD negative status showed 548 cases(97.16%)as RhD negative and 16 cases(2.84%)as RhD variants.The distribution of Rh phenotypes was ccee,Ccee,ccEe,CCee,CcEe and CCEe.The RhCE phenotypes of RHD variants all contained C or E antigens and there were several alleles which were previously reported.To be specific,DEL RHD1227A homozygous,weak D15,weak D15/DEL RHD1227A,DVI III,RHD-CE(5)-D and RHD-CE(5)-D/711delC were detected in 2 cases,6 cases,3 cases,1 case,3 cases and 1 case respectively.Conclusion The most common RhCE phenotype of RhD negative donors were ccee and Ccee.There was polymorphism in RHD gene with D variant,weak D15 and DEL RHD1227A were the most common.The RhCE phenotype of D variant was associated with RHD genotype.关键词
RhD阴性/RhCE表型/D变异型Key words
RhD negative/RhCE phenotype/D variant分类
医药卫生引用本文复制引用
张霞,赵高伟,冯智慧,郭明..RhD初筛阴性献血者RhCE表型分布和D变异型分子遗传背景研究[J].医学新知,2025,35(11):1279-1285,7.基金项目
临沂市重点研发计划(医学类)项目(2022YX0110) （医学类）
