中国草地学报2025，Vol.47Issue(11)：1-14,14.DOI:10.16742/j.zgcdxb.20240469
花苜蓿HSF基因家族鉴定及其对干旱胁迫的响应分析
Identification of HSF Gene Family in Medicago ruthenica and Analysis of Their Response to Drought Stress
摘要
Abstract
Heat shock transcription factors(HSFs)play crucial roles in plant growth and development,as well as in responses to biotic and abiotic stresses.In this study,we identified the MruHSF gene family in Medicago ruthenica using genomic data and conducted bioinformatic analyses.The expression patterns of MruHSF gene under drought stress were comprehensively analyzed through transcriptomic data and quantitative real-time PCR(qRT-PCR),preliminarily exploring their potential roles in drought stress responses.The results showed that 27 MruHSF family members,with encoded proteins ranging from 97 to 505 amino acids(aa),isoelectric points(pI)between 4.64 and 8.72,and molecular weights of 10 998.3 to 55 296.45 Da.Except for three unassigned gene,the remaining MruHSF genes were distributed across eight chromosomes.Phylogenetic analysis classified them into three evolutionary clades.Conserved motif analysis revealed that all MruHSF proteins except MruHSF02,MruHSF03,and MruHSF27 contained Motifs 1,2,and 4,which were highly conserved in M.ruthenica HSF protein.Additionally,multiple cis-acting elements related to hormone response,light response,and abiotic stress were identified in the pro-moter regions of MruHSF genes.Expression analysis shows that MruHSF01,MruHSF08,MruHSF13,MruHSF16,and MruHSF22 exhibited significant differential expression under drought stress,suggesting their potential roles in the drought response mechanism of M.ruthenica.关键词
花苜蓿/HSF基因家族/干旱胁迫/基因表达分析Key words
Medicago ruthenica/HSF gene family/Drought stress/Expression analysis分类
农业科技引用本文复制引用
贺诗淼,李彭真,王召明,刘志鹏,周强..花苜蓿HSF基因家族鉴定及其对干旱胁迫的响应分析[J].中国草地学报,2025,47(11):1-14,14.基金项目
内蒙古自治区"揭榜挂帅"项目(2022JBGS0040) （2022JBGS0040）
2023年国家草业技术创新中心(筹)重大创新平台建设专项(CCPTZX2023N04) （筹）
国家自然科学基金项目(32201444) （32201444）
