中国草地学报2025，Vol.47Issue(11)：28-37,10.DOI:10.16742/j.zgcdxb.20250131
基于转录组分析紫羊茅根系对盐胁迫的响应
Transcriptomic Analysis of Root Responses to Salt Stress in Festuca rubra
摘要
Abstract
Salt stress is one of the most critical abiotic factors limiting plant growth and development.An in-depth understanding of the molecular mechanisms underlying salt tolerance in forage grasses is essen-tial for the improvement of saline-alkali soils and the breeding of salt-resistant varieties.In this study,Fes-tuca rubra L.was subjected to a series of salt concentration gradients to determine the concentration most sensitive to root response,which was identified as 100 mmol/L NaCl.Based on this,F.rubra roots exposed to 100 mmol/L NaCl were analyzed using RNA-Seq technology to elucidate transcriptomic changes under salt stress.A total of 43 091 differentially expressed genes(DEGs)were identified com-pared with the control group,including 16 486 significantly upregulated and 26 605 significantly downregu-lated genes.Gene Ontology(GO)enrichment analysis revealed that these DEGs were primarily enriched in intracellular signal transduction,protein transport,cytosol and GTPase activity.KEGG enrichment analysis showed that the DEGs were mainly involved in metabolic pathways such as the mitogen-activated protein kinase(MAPK)signaling pathway,endoplasmic reticulum protein processing,and the tricarbox-ylic acid(TCA)cycle.Within the MAPK signaling pathway,genes encoding MAPKs related to salt stress resistance were upregulated in F.rubra roots.Moreover,several transcription factor families,including C2H2,bHLH,NAC,MYB,WRKY,and bZIP,exhibited differential expression under salt stress,with members of the C2H2 family showing particularly strong responses.In conclusion,under 100 mmol/L NaCl stress,F.rubra roots primarily respond by upregulating MAPK gene expression,activating the MAPK signaling pathway,and regulating the activity of key transcription factors to enhance osmotic adjustment and salt tol-erance.关键词
紫羊茅/盐胁迫/根系/转录组/MAPK信号通路Key words
Festuca rubra/Salt stress/Root systems/Transcriptome/MAPK signaling pathway分类
农业科技引用本文复制引用
郁文睿,景雨晴,唐钰展,韩佳彤,郭倩倩..基于转录组分析紫羊茅根系对盐胁迫的响应[J].中国草地学报,2025,47(11):28-37,10.基金项目
北京林业大学大学生创新创业训练计划项目(202510022485) （202510022485）
