中国草地学报2025，Vol.47Issue(11)：82-94,13.DOI:10.16742/j.zgcdxb.20250006
紫云英种带真菌多样性研究
Research on Seed-Borne Fungal Diversity of Astragalus sinicus
摘要
Abstract
As an important leguminous green manure crop,Astragalus sinicus plays a crucial role in improv-ing soil physicochemical properties and enhancing crop yield and quality.To investigate the diversity of seed-borne fungi associated with A.sinicus,this study conducted isolation and identification analyses on seeds from three varieties:Xiangzi No.1(XZ),Xinzi No.2(HX),and Yijiangzi(YJ).Using traditional isolation and culture methods,a total of 13 fungal species belonging to 13 genera were identified.Among them,Penicillium sp.was predominant,accounting for 42.5%,27.0%,and 17.5%of isolates from XZ,HX,and YJ,respec-tively.3 species were uniquely isolated from XZ and HX,and Fusarium oxysporum and Mucor circinelloides from both XZ and YJ.High-throughput sequencing showed that the dominant fungal taxa associated with the seeds of the three A.sinicus varieties were Ascomycota,Basidiomycota,and unclassified fungi,collectively comprising 90.29%of the total fungal community.A total of 72,53,and 64 genera were detected in XZ,HX,and YJ,respectively,with Alternaria and Fusarium being among the common genera.Notably,several gen-era identified through high-throughput sequencing(Penicillium,Fusarium,Chaetomium,and Cladosporium)were also detected by traditional culture methods,validating the consistency between the two approaches.Com-pared with traditional isolation,high-throughput sequencing greatly expanded the detected diversity of seed-associated fungal.In conclusion,A.sinicus seeds harbor a highly diverse fungal community,including common pathogenic genera such as Penicillium,which can cause storage mold,and Alternaria,which is known to cause field diseases.关键词
绿肥/种带微生物/高通量测序/多样性/致病性Key words
Green manure/Seed-borne microorganisms/High-throughput sequencing/Diversity/Pathogenicity分类
农业科技引用本文复制引用
杨吉明,申友磊,郑荣春,段廷玉,古丽君..紫云英种带真菌多样性研究[J].中国草地学报,2025,47(11):82-94,13.基金项目
国家绿肥产业技术体系项目(CARS-22) （CARS-22）
