中国人兽共患病学报2025，Vol.41Issue(10)：1005-1010,1015,7.DOI:10.3969/j.issn.1002-2694.2025.00.166
PE_PGRS37蛋白通过抑制巨噬细胞自噬流促进结核分枝杆菌的胞内定殖
PE_PGRS37 protein promotes intracellular colonization of Mycobacterium tuberculosis by inhibiting macrophage autophagy flow
摘要
Abstract
This study explored the effect of the Mycobacterium tuberculosis(Mtb)PE/PPE family protein PE_PGRS37 on the growth of Mycobacterium smegmatis(Ms)and macrophage autophagy during Mtb infection.The pe_pgrs37 gene was amplified from Mtb genome through PCR,and the recombinant vector pAIN-PE_PGRS37 was successfully constructed through homologous recombi-nation.pAIN-PE_PGRS37 and pAIN were integrated into Ms through electroshock to construct pAIN-PGRS37/Ms and pAIN/Ms re-combinant bacteria.Western blotting indicated that the PE_PGRS37 protein was correctly expressed in pAIN-PE_PGRS37/Ms.The re-combinant bacteria were inoculated in 7H9/7H10 medium,and their colony morphology and growth curves were observed.No signifi-cant difference in colony morphology was observed between pAIN-PE_PGRS37/Ms and pAIN/Ms.The growth rate significantly in-creased between 10 and 16 h,and a plateau was reached at 26 h.After infection of U937 cells with pAIN-PE_PGRS37/Ms and pAIN/Ms,macrophage autophagy flow was detected with western blotting and immunofluorescence.In the pAIN-PE_PGRS37/Ms-infected group,compared with the pAIN/Ms-infected group,macrophage LC3-II and p62 protein expression was significantly up-regulated(P<0.001)and inhibited autophagosome and lysosome fusion.The intracellular survival of the recombinant bacteria was detected through colony counting,and pAIN-PE_PGRS37/Ms showed significantly greater survival in macrophages at 12 h,24 h,and 48 h than pAIN/Ms(P<0.05).Our results suggested that PE_PGRS37 protein promotes Mycobacterium survival in macrophages by blocking macro-phage autophagy flow,thus inhibiting macrophage autophagy.关键词
结核分枝杆菌/自噬/PE/PPE家族蛋白/PE_PGRS37蛋白Key words
Mycobacterium tuberculosis/autophagy/PE/PPE family protein/PE_PGRS37分类
医药卫生引用本文复制引用
李梦雨,崔莹莹,党光辉,刘思国,张镇钧,冯婷婷,王慧,聂蝉蝉,陈春文,高云洁,段祎凡,郭若男..PE_PGRS37蛋白通过抑制巨噬细胞自噬流促进结核分枝杆菌的胞内定殖[J].中国人兽共患病学报,2025,41(10):1005-1010,1015,7.基金项目
十四五国家重点研发计划(No.2021YFD1800403) （No.2021YFD1800403）
国家自然科学基金(No.32273005) （No.32273005）
中央级公益性科研院所基本科研业务费专项(No.1610302024002) 14th Five-Year National Key Research and Development Program(No.2021YFD1800403) （No.1610302024002）
National Natural Science Foundation of China(No.32273005) （No.32273005）
Special Fund for Basic Scientific Research Operations of Public Welfare Research Institutes at the Central Level(No.1610302024002) （No.1610302024002）
