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ncBCG201对BCG适应逆境及胞内生存能力的影响

杜泽锦章恺伦范玲刘晗郭爱珍陈颖钰

中国人兽共患病学报2025，Vol.41Issue(10)：1011-1015,5.

中国人兽共患病学报2025，Vol.41Issue(10)：1011-1015,5.DOI:10.3969/j.issn.1002-2694.2025.00.161

ncBCG201对BCG适应逆境及胞内生存能力的影响

Effects of ncBCG201 on BCG stress adaptation and intracellular survival

杜泽锦 ¹章恺伦 ¹范玲 ¹刘晗 ¹郭爱珍 ²陈颖钰³

作者信息

1. 农业微生物资源发掘与利用全国重点实验室,动物科技学院动物医学院,华中农业大学,武汉 430070
2. 农业微生物资源发掘与利用全国重点实验室,动物科技学院动物医学院,华中农业大学,武汉 430070||国家动物结核病专业实验室(武汉),武汉 430070||洪山实验室,武汉 430070
3. 农业微生物资源发掘与利用全国重点实验室,动物科技学院动物医学院,华中农业大学,武汉 430070||国家动物结核病专业实验室(武汉),武汉 430070
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摘要

Abstract

This study investigated the effect of small RNA ncBCG201 on the stress adaptation and intracellular survival of Myco-bacterium bovis.Differentially expressed ncRNAs were screened with RNA-seq after BCG infection of THP-1 macrophages.The ncBCG201 overexpression strain was constructed,and its stress survival,growth,biofilm formation,and intracellular survival were assessed.The ncBCG201 strain showed slower growth and earlier entry into stationary phase.Survival significantly increased under car-bon starvation but decreased under membrane pressure.Biofilm formation capability decreased.Intracellular survival in THP-1 macro-phages at 24 h and 48 h was significantly higher than observed in the control.Therefore,ncBCG204 enhances BCG survival within macrophages by modulating stress adaptability.

关键词

卡介苗/结核分枝杆菌/sRNA/ncBCG201/逆境适应

Key words

Bacillus Calmette Guerin(BCG)/Mycobacterium tuberculosis(M.tuberculosis)/sRNA/ncBCG201/stress adapta-tion

分类

医药卫生

引用本文复制引用

杜泽锦,章恺伦,范玲,刘晗,郭爱珍,陈颖钰..ncBCG201对BCG适应逆境及胞内生存能力的影响[J].中国人兽共患病学报,2025,41(10):1011-1015,5.

基金项目

National Natural Science Foundation of China(No.U22A20525)国家自然科学基金区域创新发展联合基金(No.U22A20525) （No.U22A20525）

中国人兽共患病学报

OA北大核心

ISSN：1002-2694

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