中国人兽共患病学报2025，Vol.41Issue(10)：1016-1024,9.DOI:10.3969/j.issn.1002-2694.2025.00.162
靶向高通量测序在快速检测分枝杆菌中的应用
Efficacy of targeted next-generation sequencing in the rapid detection of Mycobacterium
摘要
Abstract
The purpose of this study was to evaluate the application value of targeted next-generation sequencing(tNGS)for rapid detection of Mycobacterium tuberculosis.From September 2021 to October 2024,1 178 clinical samples from hospitalized pa-tients at the Non-tuberculous Mycobacteria Disease Diagnosis and Treatment Center of Guangzhou Chest Hospital were collected,in-cluding 272 sputum samples,871 broncho alveolar lavage fluid(BALF)samples,and 35 other samples.These samples were analyzed with tNGS and the liquid culture+DNA microarray chip method(referred to as the"culture identification method"),and the detection results between methods were compared.The numbers of Mycobacterium tuberculosis complex(MTBC)samples identified with tNGS and the culture identification method were 172 and 127,respectively,and the numbers of non-tuberculous mycobacteria(NTM)samples detected were 517 and 474,respectively.The detection rate of Mycobacterium was 56.88%with tNGS and was significantly lower(49.49%)with the culture identification method(χ2=13.27,P<0.05).For NTM identification,when sputum was used,the detection rate of tNGS was higher than that of the culture identification method(χ2=24.14,P<0.05).However,when BALF was used,no significant difference in detection rates was observed between tNGS and the culture identification method(χ2=3.97,P=0.06).When different sample types from the same patient were analyzed with tNGS for NTM,BALF was found to be a better choice than sputum(χ2=4.05,P<0.05).However,with the culture identification method,we observed no significant difference between sample types(χ2=2.72,P=0.10).Furthermore,both the MTBC and NTM sequences detected with tNGS were significantly higher in the culture-positive group than the culture-negative group.With increasing sequence numbers,the proportion of cases in the culture-positive group progressively rose,whereas an inverse trend was observed in the culture-negative group.With clinical diagno-sis as the reference standard,the sensitivity,specificity,and Kappa value of tNGS and the culture identification method in identifying mycobacterial diseases were 85.41%,91.32%,0.74 and 74.73%,93.15%,0.63,respectively.Compared with the culture identifica-tion method,tNGS for rapid detection of Mycobacterium exhibited excellent sensitivity,specificity and consistency,and its timeliness should help clinicians make precise individualized treatment plans earlier.关键词
靶向高通量测序/分枝杆菌/非结核分枝杆菌/结核分枝杆菌/培养菌鉴法Key words
targeted next-generation sequencing/Mycobacterium/non-tuberculous mycobacteria/Mycobacterium tuberculosis/culture identification method分类
医药卫生引用本文复制引用
琚岱晨,林佳敏,陈华,陈品儒,苏碧仪,谭耀驹,胡锦兴,朱家楼..靶向高通量测序在快速检测分枝杆菌中的应用[J].中国人兽共患病学报,2025,41(10):1016-1024,9.基金项目
广州市医学重点学科(No.2025-2027年) （No.2025-2027年）
广州市科技计划项目(No.2023A03J0991、No.2024A03J0437、No.2024B03J1345) Guangzhou Medical Key Discipline(No.2025-2027) （No.2023A03J0991、No.2024A03J0437、No.2024B03J1345）
Science and Technology Program of Guangzhou(No.2023A03J0991,No.2024A03J0437,No.2024B03J1345) （No.2023A03J0991,No.2024A03J0437,No.2024B03J1345）
