中国人兽共患病学报2025，Vol.41Issue(10)：1073-1080,8.DOI:10.3969/j.issn.1002-2694.2025.00.171
消化道疾病患者感染肝螺杆菌和幽门螺杆菌的检测
Detection of Helicobacter pylori and Helicobacter hepaticus in patients with digestive tract diseases
摘要
Abstract
This study was aimed at investigating the presence of Helicobacter hepaticus(Hh)infection in patients with digestive tract diseases and evaluating Helicobacter pylori(Hp)infection status in patients with digestive tract cancers other than gastric cancer.Fecal samples were collected from 197 patients with digestive tract diseases at the Affiliated Cancer Hospital of Guizhou Medical Uni-versity and from 149 healthy volunteers residing in Guiyang.Hp stool antigen(HpSA)was detected with the colloidal gold method.Af-ter the extraction of fecal DNA,the Hp specific ureA gene and the Hh specific 16S rRNA gene were amplified via nested PCR,and the amplified products were subsequently confirmed through sequencing analysis.The study included 197 patients with digestive system diseases,comprising 135 cases of colorectal cancer,32 cases of chronic gastritis,22 cases of gastric cancer,5 cases of liver cancer,and 3 cases of cholangiocarcinoma.The detection rate of HpSA was 31.5%(62/197).HpSA was detected across all five disease catego-ries,and the highest detection rate was observed in patients with gastric cancer,at 50.0%(11/22),or colorectal cancer,at 24.4%(33/135).The positivity rate of Hp ureA gene PCR was 7.6%(15/197),and sequencing confirmed that the amplified products were in-deed Hp ureA gene fragments.Notably,the highest detection rate was observed in patients with colorectal cancer,at 8.9%(12/135).The positivity rate of Hh 16S rRNA gene PCR was 11.2%(22/197),and sequencing confirmed that the amplified products were in-deed Hh 16S rRNA gene fragments.Hh 16S rRNAgene presence was detected in patients with all five diseases,and the highest detec-tion rate was observed in patients with colorectal cancer,at 11.1%(15/135).Among 149 healthy volunteers,the detection rate of HpSA was 11.4%(17/149),only one case tested positive for the Hp ureA gene,and the Hh 16S rRNA gene was undetectable in all samples.In conclusion,Hh infection was detected in patients with digestive tract diseases.Beyond patients with gastric cancer,the prevalence of Hp infection was also notably high among patients with colorectal cancer,liver cancer,and cholangiocarcinoma.Further investigation is warranted to elucidate the roles of the two species of Helicobacter in the occurrence and progression of digestive tract cancers.关键词
幽门螺杆菌/肝螺杆菌/消化道疾病/结直肠癌Key words
Helicobacter pylori/Helicobacter hepaticus/digestive tract disease/colorectal cancer分类
医药卫生引用本文复制引用
许晓丽,李其泽,罗婷婷,孙建超,罗庆,王燕,张维,刘琦,陈峥宏..消化道疾病患者感染肝螺杆菌和幽门螺杆菌的检测[J].中国人兽共患病学报,2025,41(10):1073-1080,8.基金项目
贵州省重点实验室(No.ZDSYS[2023]004) （No.ZDSYS[2023]004）
国家科技部及教育部高等学校学科创新引智计划(No.D20009) （No.D20009）
贵州省中央引导地方科技发展资金项目(No.黔科合中引地[2025]024) Guizhou Key Laboratory(No.ZDSYS[2023]004) （No.黔科合中引地[2025]024）
The High-Level Talent Introduction Plan for Discipline Innovation in Higher Education Institutions(The 111 Project)(No.D20009) （The 111 Project）
Central-Guided Local Science and Technology Projects of Guizhou Province(No.Qiankehe[2025]024) （No.Qiankehe[2025]024）
