中国畜牧兽医2025，Vol.52Issue(12)：5539-5548,10.DOI:10.16431/j.cnki.1671-7236.2025.12.002
miR-200b生物学功能及其对小鼠卵泡发育的影响
Biological Functions of miR-200b and Its Effects on Follicle Development in Mice
摘要
Abstract
[Objective]This study aimed to explore the biological functions of miR-200b and investigate the effect on follicle development in mice.[Method]In this study,the mature sequences of miR-200b in human,mice,rat,zebrafish,pig and sheep were obtained from the NCBI database,the sequence analysis was carried out by the online software WebLogo,the miR-200b target genes prediction was carried out by TargetScan and miRDB,and the GO function and KEGG pathway enrichment analysis of these target genes were performed.The miR-200b antagonistic model was constructed by intraperitoneal injection of miRNA antagomir,and blood and samples from various tissues and organs of the mice were collected.The expression level of miR-200b in ovaries was detected by Real-time quantitative PCR.The organ coefficient was counted,the morphological changes of ovarian tissues were observed by HE staining,the follicles were counted,and the serum E2 level was detected by ELISA.[Result]The seed sequences of miR-200b were completely identical across different species,and there were differences in the last 1-2 bases of the mature sequence.A total of 607 target genes were predicted by TargetScan and miRDB software,which were mainly enriched in GO entries such as transcriptional regulation,DNA and protein binding,as well as KEGG signaling pathways such as PI3K-Akt,MAPK,longevity regulation,and estrogen.Compared with control and antagomir NC groups,the expression of miR-200b in ovary of mice in miR-200b antagonist group was extremely significantly downregulated(P＜0.01),the ovarian index and the number of pre-antral follicles were extremely significantly decreased(P＜0.01),and the number of antral follicles was extremely significantly increased(P＜0.01).There was also an increase in follicle volume and granulosa cell number.The serum E2 level was extremely significantly increased(P＜0.01).[Conclusion]miR-200b negatively regulated the growth of granulosa cells and follicle development in mice,which provided new evidence for an in-depth analysis of the regulatory function of miR-200b on follicle development.关键词
小鼠/miR-200b/生物学功能/卵泡发育Key words
mice/miR-200b/biological function/follicle development分类
农业科技引用本文复制引用
宋鹏琰,锡建中,陈晓勇,方翟,邢凤,周荣艳..miR-200b生物学功能及其对小鼠卵泡发育的影响[J].中国畜牧兽医,2025,52(12):5539-5548,10.基金项目
国家自然科学基金青年基金(32402760) （32402760）
塔里木大学校长基金(TDZKBS202410) （TDZKBS202410）
