中国畜牧兽医2025，Vol.52Issue(12)：5549-5559,11.DOI:10.16431/j.cnki.1671-7236.2025.12.003
月桂酸甘油酯对奶牛体外瘤胃发酵参数和甲烷产量的影响
Effects of Glycerol Monolaurate on in vitro Rumen Fermentation Parameters and Methane Production in Dairy Cows
摘要
Abstract
[Objective]Glycerol monolaurate(GML)is a monoglyceride formed by esterification of lauric acid and glycerol.It possesses antimicrobial activity and haspotential as a feed additive to improve animal feed efficiency.This study aimed to investigate the effects of different dosages of GML on in vitro rumen fermentation parameters and methane production in dairy cows.[Method]An in vitro fermentation technique was employed using a total mixed ration(TMR)with a concentrate-to-forage ratio of 4∶6 as the substrate.Four GML supplementation levels(0(control group),0.6％,1.2％,and 1.8％)were tested,with five replicates per treatment.The gas production was measured at 0,6,12,24 and 48 h of fermentation.Rumen fluid and gas samples was collected at 6,12,24 and 48 h for analysis gas production,gas component proportions and yields,and rumen fluid fermentation parameters.[Result]①Compared with control group,the gas production in all GML-supplemented groups at 6 h of fermentation was significantly decreased(P＜0.05).The gas production at 6 and 48 h of fermentation showed a significant linear change with the increase in GML addition(P＜0.05).②Compared with control group,the proportions of methane,hydrogen and carbon dioxide were not affected by GML addition at any time point(P＞0.05).The methane yield in 0.6％and 1.2％GML groups at 6 h of fermentation was significantly decreased(P＜0.05).Regarding gas yields,methane,hydrogen and carbon dioxide at 6 h of fermentation,and carbon dioxide yield at 48 h of fermentation,showed a significant quadratic change with the increase in GML addition(P＜0.05).③Compared with control group,the proportions of total volatile fatty acid(TVFA),acetic acid and propionic acid and A/P in rumen fluid of all GML-supplemented groups showed no significant difference at different time points(P＞0.05).The proportion of butyric acid at 6 h of fermentation exhibited a significant quadratic change(P＜0.05).④Compared with control group,the ammonia nitrogen(NH3-N)concentration at 6 h of fermentation in 1.2％GML group was significantly increased(P＜0.05).The NH3-N concentration at 12 h of fermentation in 1.2％GML group was significantly decreased(P＜0.05),while in 1.8％GML group was significantly increased(P＜0.05).The NH3-N concentration at 24 h of fermentation in all GML-supplemented group was significantly increased(P＜0.05).There was no significant difference of pH among all groups at any time point(P＞0.05).The NH3-N concentration at 12 h of fermentation exhibited a significant quadratic change(P＜0.05).[Conclusion]Under the conditions of this study,supplementation with 0.6％GML significantly reduced cumulative gas production,methane yield,and NH3-N concentration during 6-12 h of fermentation,without significantly affecting VFA composition and pH,indicating that GML had the potential to modulate rumen fermentation processes and inhibit methane production at appropriate supplementation level,providing a theoretical basis for the development of novel rumen methane-reducing additives.关键词
奶牛/月桂酸甘油酯/瘤胃/体外发酵/甲烷减排Key words
dairy cows/glycerol monolaurate/rumen/in vitro fermentation/methane emission reduction分类
农业科技引用本文复制引用
王晓涵,吴慧光,任立敬,萨吉代姆·玉苏甫江,林淼,赵静雯..月桂酸甘油酯对奶牛体外瘤胃发酵参数和甲烷产量的影响[J].中国畜牧兽医,2025,52(12):5549-5559,11.基金项目
江苏省研究生实践创新计划项目(SJCX23-1996) （SJCX23-1996）
江苏省碳达峰与碳中和科技创新专项(BE2022309) （BE2022309）
国家现代农业产业技术体系(CARS-36) （CARS-36）
