中国畜牧兽医2025，Vol.52Issue(12)：5591-5599,9.DOI:10.16431/j.cnki.1671-7236.2025.12.006
硒代蛋氨酸对呕吐毒素诱导的兔回肠损伤的保护作用
Protective Effect of Selenomethionine on Ileal Injury Caused by Deoxynivalenol in Rabbits
摘要
Abstract
[Objective]This study aimed to explore the effect of adding selenomethionine(SeMet)to the rabbit diet on the oxidative damage and inflammatory damage of the ileum caused by deoxynivalenol(DON),providing a new idea for the treatment of DON poisoning diseases.[Method]Forty 35-day-old IRA rabbits were randomly divided into 4 groups:The blank control(CON),model(DON),SeMet and DON+SeMet groups,with 10 rabbits in each group.From the first day of the experiment,rabbits in SeMet and DON+SeMet groups were fed 0.2 mg/kg SeMet every day for 21 days.On the 15th day,rabbits in DON and DON+SeMet groups were given 0.5 mg/kg BW of DON by gavage every day for 7 days.On the 21 st day of the experiment,after 24 h of challenge,the ileal tissues of each group were collected.Through HE and PAS staining,as well as scanning electron microscopy,the morphology of the intestinal villi,crypts,the number of goblet cells,and the ultrastructure changes were observed.The levels of oxidative stress-related indicators and inflammatory factors in ileum were detected by ELISA.The expression levels of inflammatory-related genes in ileum were detected by Real-time quantitative PCR.[Result]Compared with CON group,a small number of intestinal villi in ileum of DON group were broken,and the villus height and the ratio of villus height to crypt depth were significantly decreased(P＜0.05),the crypt depth was significantly increased(P＜0.05),and the number of goblet cells was significantly reduced(P＜0.05).The results of scanning electron microscopy showed that in DON group,the surface of the ileal villi was atrophied,presenting a granular appearance,and the microvilli were detached and broken.Compared with CON group,the content of interleukin-6(IL-6)in ileum of DON group was significantly increased(P＜0.05),and the activity of glutathione peroxidase(GSH-Px)was significantly decreased(P＜0.05).The other inflammatory factors(IL-10,IL-1β and tumor necrosis factor-α(TNF-α))and oxidative stress-related indicators(malondialdehyde(MDA),total superoxide dismutase(T-SOD)and total antioxidant capacity(T-AOC))showed no significant differences(P＞0.05).Compared with DON group,the addition of SeMet to the diet could significantly improve the intestinal pathological damage induced by DON,and the content IL-6 was significantly reduced(P＜0.05).[Conclusion]DON could induce inflammatory responses in rabbits and damage the villi structure of the ileum.Meanwhile,SeMet could alleviate the villi rupture and inflammatory damage caused by DON in rabbits.The experimental results provided morphological data and theoretical basis for the intervention of SeMet in the intestinal damage induced by DON,and offered new ideas and methods for the prevention and treatment of DON poisoning diseases.关键词
硒代蛋氨酸(SeMet)/呕吐毒素(DON)/兔/回肠损伤Key words
selenomethionine(SeMet)/deoxynivalenol(DON)/rabbit/ileal injury分类
农业科技引用本文复制引用
位兰,张梦雨,崔敏,张自强,朱雪敏,刘玉梅,吕琼霞,陈晓光..硒代蛋氨酸对呕吐毒素诱导的兔回肠损伤的保护作用[J].中国畜牧兽医,2025,52(12):5591-5599,9.基金项目
校企合作科研项目(20240012) （20240012）
