中国畜牧兽医2025，Vol.52Issue(12)：5600-5613,14.DOI:10.16431/j.cnki.1671-7236.2025.12.007
转录组学和代谢组学技术在牛肉质性状研究中的应用
Application of Transcriptomics and Metabolomics Technologies in Beef Quality Traits Research
摘要
Abstract
With the continuous improvement of living standards,consumers' concern regarding beef quality has been increasing.Transcriptomics and metabolomics,with their advantages of high-throughput,high sensitivity,and systematicity,have become powerful tools for studying beef quality traits.Transcriptomics,through RNA-sequencing(RNA-Seq)technology combined with bioinformatics methods,has revealed the relationship between gene expression patterns and meat quality traits.Metabolomics focuses on endogenous small molecules with molecular weights below 1 500 u within biological systems.It employs techniques such as nuclear magnetic resonance(NMR),gas chromatography-mass spectrometry(GC-MS),and liquid chromatography-mass spectrometry(LC-MS)to detect metabolites,and utilizes multivariate analysis,machine learning(ML),and functional analysis to investigate the correlation between metabolite abundance and meat quality traits.The integrated analysis of transcriptomics and metabolomics can study the relationship between gene expression patterns and metabolite abundance by integrating differentially expressed genes and differentially abundant metabolites.Methods such as gene-metabolite networks and shared KEGG pathway enrichment between the two can comprehensively elucidate the intrinsic differences in meat quality formation and the molecular mechanisms underlying complex traits.This review systematically summarizes the technical principles and workflows of transcriptomics and metabolomics,as well as their current applications in beef quality research.It also discusses the challenges faced by current research and the directions for future development,aiming to provide scientific references for screening molecular breeding markers for high-quality beef cattle and formulating precise nutritional intervention strategies.关键词
转录组学/代谢组学/联合分析/肉品质Key words
transcriptomics/metabolomics/integrated analysis/meat quality分类
农业科技引用本文复制引用
志莉,戴思凡,刘雪琴,陈泉贞,俞英,毛华明..转录组学和代谢组学技术在牛肉质性状研究中的应用[J].中国畜牧兽医,2025,52(12):5600-5613,14.基金项目
云南省鹤庆县奶牛产业科技特派团(202204BI090005) （202204BI090005）
