中国畜牧兽医2025，Vol.52Issue(12)：5614-5628,15.DOI:10.16431/j.cnki.1671-7236.2025.12.008
不同周龄黑羽番鸭肠道微生物变化规律研究
Study on the Change Pattern of Gut Microbiota in Black-feathered Muscovy Ducks at Different Weeks of Age
摘要
Abstract
[Objective]This study aimed to investigate the composition and distribution characteristics of cecal microbiota in Black-feathered Muscovy ducks(Cairina moschata)during growth and development at different weeks of age.[Method]Experimental subjects included Black-feathered Muscovy ducks at 3,5,7,9,11,13,and 43 weeks of age,with 5 males and 5 females randomly selected for each age of groups.Cecal content samples of Black-feathered Muscovy ducks were subjected to put on 16S rRNA high-throughput sequencing,with analysis performed through QIIME2 and EasyAmplicon software.Microbial community characteristics were evaluated using LEfSe,STEM,PICRUSt2,and Bugbase platforms for microbiota classification and functional enrichment analysis.[Result]The diversity analysis results showed that there were significant differences in the cecal microbiota of Black-feathered Muscovy ducks among 3,5,7,13,and 43 weeks of age(P＜0.05).However,a significant difference in Ace index was only observed between male and female Black-feathered Muscovy ducks at 13 weeks of age(P＜0.05).The analysis results of the composition of cecal contents showed that the core microbiota at the phylum level in Black-feathered Muscovy ducks at different weeks of age included Bacteroidota(53.77％),Firmicutes(26.92％),and Desulfobacterota(5.38％).The known core microbiota at the genus level included Bacteroides(27.47％),Rikenellaceae_RC9_gut_group(5.88％),Desulfovibrio(5.21％),and Methanobrevibacter(3.46％).Further heat map analysis of the microbial communities with the top 30 average abundances at the genus level showed significant differences of cecal microbiomes in Black-feathered Muscovy ducks between 3 and 13-43 weeks of age.COG and KEGG functional enrichment analysis indicated that the cecal microbiota of Black-feathered Muscovy ducks during growth and development stages were predominantly associated with metabolic processes and digestive system functions.[Conclusion]The cecal microbiotas of Black-feathered Muscovy ducks were predominantly composed of Bacteroidota,Firmicutes,and Desulfobacterota,with dynamic structural and abundance changes occurring at developmental stages to meet physiological demands.Alloprevotella and UCG-008 of the Ruminococcaceae family were candidate key regulatory bacterial genera for the growth and development regulation process of Black-feathered Muscovy ducks at 3-13 weeks of age.The results provided new insights into the effect of gut microbiota on the growth and development characteristics of Black-feathered Muscovy ducks.关键词
黑羽番鸭/16S rRNA测序/肠道菌群/生长发育Key words
Black-feathered Muscovy duck/16S rRNA sequencing/gut microbiota/growth and development分类
农业科技引用本文复制引用
陆艳凤,张蕾,段修军,江正梅,孙国波..不同周龄黑羽番鸭肠道微生物变化规律研究[J].中国畜牧兽医,2025,52(12):5614-5628,15.基金项目
江苏省科技重点研发项目(BE2022359) （BE2022359）
江苏农牧科技职业院校科研项目(NSF2023ZR03) （NSF2023ZR03）
