中国药房2025，Vol.36Issue(23)：2912-2917,6.DOI:10.6039/j.issn.1001-0408.2025.23.05
基于CXCL12/CXCR4/STAT3信号通路探讨肾必宁颗粒对IgA肾病大鼠的肾脏保护作用及机制
Exploration of the renal protective effect and mechanism of Shenbining granule on IgA nephropathy rats based on the CXCL12/CXCR4/STAT3 signaling pathway
摘要
Abstract
OBJECTIVE To investigate the renal protective effect and mechanism of Shenbining granule on IgA nephropathy(IgAN)rats by regulating the CXC chemokine motif ligand 12(CXCL12)/CXC chemokine receptor 4(CXCR4)/signal transducer and activator of transcription 3(STAT3)signaling pathway.METHODS A total of 60 rats were randomly assigned into blank group(n=12)and modeling group(n=48).IgAN model of modeling group was induced by using bovine serum albumin,carbon tetrachloride and lipopolysaccharide,followed by model validation.Ultimately,a total of 55 rats(9 in the blank group,46 in the modeling group)were included in the subsequent study.The rats in the modeling group were randomly divided into model group(n=10),prednisone acetate group[positive control group,6.25 mg/(kg·d),n=12],Shenbining granule low-and high-dose groups[4.17,8.33 g/(kg·d),n=12].They were given relevant medicine/distilled water intragastrically,once a day,for 4 consecutive weeks.After the last medication,biochemical indicators in the urine and serum of rats were measured,and pathological morphological changes in the renal tissues of rats were observed.IgA deposition in the renal tissues,as well as the mRNA expression levels of CXCL12,CXCR4 and STAT3,and the protein expression levels of CXCL12,CXCR4,STAT3 and phosphorylated STAT3(p-STAT3)were detected.Additionally,the level of interleukin-6(IL-6)in the renal tissue was measured.RESULTS Compared with the model group,the low-dose and high-dose Shenbining granule groups showed significantly decreased urinary red blood cell count,24 h total urinary protein,blood urea nitrogen,serum creatinine,and alanine amino-transferase,along with increased Alb levels(P＜0.05).Pathological damage in the renal tissues was alleviated,with reduced IgA deposition in the mesangial region(P＜0.05);protein and mRNA expressions of CXCL12,CXCR4 and STAT3,as well as phosphorylation level of STAT3 protein and the IL-6 level,were significantly decreased in renal tissue(P＜0.05).CONCLUSIONS Shenbining granule may exert its renal protective effects in IgAN rats by inhibiting the activation of the CXCL12/CXCR4/STAT3 signaling pathway,downregulating the expression of inflammatory factors such as IL-6,alleviating renal inflammation,and thereby improving renal pathological damage.关键词
肾必宁颗粒/IgA肾病/趋化因子/CXCL12/CXCR4/STAT3信号通路/白细胞介素6Key words
Shenbining granule/IgA nephropathy/chemokine/CXCL12/CXCR4/STAT3 signaling pathway/interleukin-6分类
医药卫生引用本文复制引用
王旭,宋纯东,陈晨晨,姜浩然..基于CXCL12/CXCR4/STAT3信号通路探讨肾必宁颗粒对IgA肾病大鼠的肾脏保护作用及机制[J].中国药房,2025,36(23):2912-2917,6.基金项目
国家自然科学基金面上项目(No.82074493) （No.82074493）
河南省卫生健康委员会国家中医药传承创新中心联合共建科研专项(No.2024ZXZX1019) （No.2024ZXZX1019）
河南省中医药科学研究专项重点课题(No.2024ZY1002) （No.2024ZY1002）
河南省中医药科学研究专项课题(No.2023ZYZD02) （No.2023ZYZD02）
