中国药房2025，Vol.36Issue(23)：2924-2929,6.DOI:10.6039/j.issn.1001-0408.2025.23.07
复方蜥蜴散诱导细胞凋亡及自噬性死亡逆转胃癌顺铂耐药的机制研究
Study on the mechanism of Compound lizard powder on reversing cisplatin resistance in gastric cancer by inducing apoptosis and autophagic death
摘要
Abstract
OBJECTIVE To study the mechanism of Compound lizard powder on reversing cisplatin(DDP)resistance in resistant gastric cancer cell MKN45/DDP through nuclear factor-κB(NF-κB)/SNAIL signaling pathway.METHODS MKN45/DDP cells were divided into model group(20%normal rat serum),DDP group(1.3 μg/mL DDP+20%fetal bovine serum)and Compound lizard powder low-and high-dose drug-containing serum+DDP groups(17.2,68.8 g/kg Compound lizard powder 20%drug-containing serum+1.3 μg/mL DDP).The viability,apoptosis and intracellular autophagosome of MKN45/DDP cells were detected.The content of microtubule-associated protein 1 light chain 3(LC3)in the cell supernatant was detected.The expressions of B-cell lymphoma 2(Bcl-2),Bcl-2-related X protein(Bax),as well as the protein expression levels of phosphorylated NF-κB p65(p-NF-κB p65)and SNAIL were detected.The molecular mechanism of Compound lizard powder in improving DDP resistance was further clarified by using NF-κB pathway agonist tumor necrosis factor-α(TNF-α).RESULTS Compared with model group and DDP group,the cell survival rates of Compound lizard powder low-and high-dose drug-containing serum+DDP groups were significantly decreased(P＜0.05),while the levels of apoptosis and autophagic death were significantly increased(P＜0.05).The expression levels of Bcl-2(except for the compound lizard powder low-dose drug-containing serum+DDP group),p-NF-κB p65 and SNAIL protein in MKN45/DDP cells were significantly decreased(P＜0.05).The content of LC3 and expression of Bax protein were significantly increased(P＜0.05).High-dose Compound lizard powder drug-containing serum+DDP could effectively reverse the down-regulation of LC3Ⅱ/LC3Ⅰ and Bax protein expression induced by TNF-α(P＜0.05).CONCLUSIONS Compound lizard powder drug-containing serum combined with DDP can induce apoptosis and autophagic death of MKN45/DDP cells by inhibiting NF-κB/SNAIL signaling pathway,thus reversing DDP resistance of cells.关键词
复方蜥蜴散/MKN45/DDP细胞/顺铂/耐药性/自噬/凋亡/NF-κB/SNAIL信号通路Key words
Compound lizard powder/MKN45/DDP cells/cisplatin/resistance/autophagy/apoptosis/NF-κB/SNAIL pathway分类
医药卫生引用本文复制引用
刘彩月,李铮,李卫强..复方蜥蜴散诱导细胞凋亡及自噬性死亡逆转胃癌顺铂耐药的机制研究[J].中国药房,2025,36(23):2924-2929,6.基金项目
国家自然科学基金项目(No.82260916) （No.82260916）
宁夏医科大学科研项目(No.XZ2023012) （No.XZ2023012）
