中国药理学通报2025，Vol.41Issue(12)：2253-2259,7.DOI:10.12360/CPB202504077
红景天苷衍生物pOBz调控Notch信号通路对缺血性脑卒中后血管生成的作用
The effect of salidroside derivative pOBz on angiogenesis after ischemic stroke by regulating Notch signaling pathway
摘要
Abstract
Aim To study the effect of p-benzoyl sali-droside(pOBz)on angiogenesis after ischemic stroke and to explore the underlying mechanism.Methods The MCAO model was prepared by suture method.Rats were divided into four groups:sham,MCAO,pOBz administration,and edaravone positive control,treated for seven days.The mNSS was used to assess the neurological impairment.Western blotting was em-ployed to detect CD31,NICD,and Hes-1 protein ex-pression,while immunofluorescence staining was ap-plies to quantify CD31-positive cells in ischemic brain tissue.In vitro an OGD/R model was established in HUVECs.Following treatment with varying pOBz con-centrations(0.01,0.1,1 μmol·L-1),the CCK-8 as-say was uses to measure cell viability,and in vitro tube formation assay was utilized to evaluate angiogenesis.Western blotting was employed again to assess CD31,NICD and Hes-1 protein levels.To further elucidate the mechanism,HUVEC were treated with the Notch inhibitor DAPT prior to grouping and pOBz administra-tion,and the same parameters were evaluated.Results pOBz significantly reduced the mNSS score of MCAO rats,increased CD31-positive cell counts,and upregu-lated CD31,NICD,and Hes-1 protein expression(P＜0.01).In vitro results further showed that pOBz could dose-dependently increase the survival rate and angio-genesis ability of HUVEC induced by OGD/R,and promote CD31,NICD and Hes-1 proteins(P＜0.01),and Notch inhibitor DAPT could reverse the above effects of pOBz.Conclusion pOBz promotes angio-genesis in HUVEC,and its mechanism involves activa-tion of the Notch signaling pathway.关键词
红景天苷衍生物pOBz/缺血性脑卒中/血小板内皮细胞分子/Notch信号通路/人脐静脉内皮细胞/血管生成Key words
salidroside derivative pOBz/ischemic stroke/platelet endothelial cell adhesion molecule/Notch signaling pathway/human umbilical vein endo-thelial cells/angiogenesis分类
医药卫生引用本文复制引用
陈景泉,江玉婷,郑雪蕊,吴慧玲,吴青青,余正双,赖文芳,洪桂祝..红景天苷衍生物pOBz调控Notch信号通路对缺血性脑卒中后血管生成的作用[J].中国药理学通报,2025,41(12):2253-2259,7.基金项目
国家自然科学基金资助项目(No 82174001) （No 82174001）
福建省自然科学基金资助项目(No 2024J01776) （No 2024J01776）
