中国中药杂志2025，Vol.50Issue(22)：6273-6280,8.DOI:10.19540/j.cnki.cjcmm.20250917.503
补肾促卵方调控CXCR4/HIF-1α信号通路促进KGN诱导血管新生改善早发性卵巢功能不全的机制研究
Mechanism of Bushen Culuan Formula in improving premature ovarian insufficiency by promoting angiogenesis induction in KGN through regulation of CXCR4/HIF-1α signaling pathway
摘要
Abstract
This study aimed to investigate the mechanism by which the Bushen Culuan Formula(BSCL)promotes angiogenesis induction in KGN via the CXCR4/HIF-1α signaling pathway.A cell model was established by treating KGN with triptolide,and based on this model,the optimal concentration of the CXCR4 inhibitor(AMD3100)was determined.CCK-8 assay was used to detect the effects of different concentrations of BSCL-medicated serum on the viability of KGN.After the optimal concentrations of each reagent were determined,KGN were divided into five groups:control group,triptolide group,AMD3100 group,BSCL group,and inhibitor group to corresponding interventions under different conditions.The migration ability,invasion ability,apoptosis rate,expression levels of intracellular related proteins,and secretion levels of pro-angiogenic factors were evaluated in each group.These results suggest that although the 10％BSCL-medicated serum could not regulate KGN through the Ang1/Tie2 pathway,it target and regulate the CXCR4/HIF-1α signaling pathway,enhance migration and invasion,significantly reduce early and total apoptosis rates,enhance the viability of granulosa cells within the follicle and promoting the high-level secretion of angiogenesis-inducing signaling factors such as CD34,VEGFA,EPO,and Ang2,to achieve the purpose of improving early-onset ovarian insufficiency caused by impaired ovarian angiogenesis.关键词
补肾促卵方/早发性卵巢功能不全/雷公藤甲素/血管新生Key words
Bushen Culuan Formula/premature ovarian insufficiency/triptolide/angiogenesis引用本文复制引用
马堃,马林纳,罗洁,高聪,范晓迪,李佳妮,高山凤,孙立华..补肾促卵方调控CXCR4/HIF-1α信号通路促进KGN诱导血管新生改善早发性卵巢功能不全的机制研究[J].中国中药杂志,2025,50(22):6273-6280,8.基金项目
中国中医科学院科技创新工程项目(CI2021A02406) （CI2021A02406）
北京市自然科学基金项目(7212193) （7212193）
孙立华名老中医工作室项目(2017-SZ-B-36) （2017-SZ-B-36）
北京中医药薪火传承"新3+3"工程示范案例项目(2023-ZYSF-21) （2023-ZYSF-21）
