中国中药杂志2025，Vol.50Issue(22)：6281-6288,8.DOI:10.19540/j.cnki.cjcmm.20250919.501
补肾促卵方调控SIRT1信号通路促进线粒体功能改善卵巢早衰的机制研究
Mechanism of Bushen Culuan Decoction in regulating SIRT1 signaling pathway to improve mitochondrial function and premature ovarian failure
摘要
Abstract
Premature ovarian failure(POF)has been a global public health concern with a rising incidence,severely affecting women's reproductive health and quality of life.This study aims to investigate the mechanisms by which Bushen Culuan Decoction(BCD)ameliorates ovarian mitochondrial dysfunction to improve POF.Fifty female BALB/c mice were randomly divided into control group,model group,positive drug group,low-,and high-dose BCD group.POF mouse model was established by subcutaneous injection of D-gal.Body weight and gonadal indices were observed,and changes in the estrous cycle were monitored.The level of FSH,E2,and LH in serum was measured using ELISA.Ovarian tissue morphology and follicle counts were assessed via HE staining.In vitro model of human ovarian granulosa cells(KGN)injury was established to quantify mitochondrial number and ROS level.Protein expression of SIRT1 and PGC-1α was analyzed using Western blot.The results showed that compared with control group,model group reduced body weight gain,disordered estrous cycles,decreased ovarian and uterine indices,elevated serum FSH and LH levels,reduced E2 level,decreased number of follicles at all developmental stages,and increased number of atretic follicles.Compared with model group,BCD group increased in ovarian and uterine indices,decreased in serum FSH and LH levels,increased in E2 level,and decreased number of atretic follicles in mice.In vitro,BCD increased mitochondrial number,reduced ROS production,and upregulated SIRT1 and PGC-1α expressions in KGN.In conclusion,BCD improves the estrous cycle,increases ovarian and uterine indices,lowers serum FSH and LH levels,elevates E2 level,reduces the number of atretic follicles,and protects ovarian function in POF mice.These effects may be associated with activation of the SIRT1 signaling pathway,thereby improving mitochondrial function and reducing oxidative stress in KGN.关键词
补肾促卵方/卵巢早衰/线粒体功能/氧化应激/沉默信息调节因子1Key words
Bushen Culuan Decoction/premature ovarian failure/mitochondrial function/oxidative stress/silencing information regulatory factor 1引用本文复制引用
马堃,李佳妮,范晓迪,马林纳,孙立华..补肾促卵方调控SIRT1信号通路促进线粒体功能改善卵巢早衰的机制研究[J].中国中药杂志,2025,50(22):6281-6288,8.基金项目
北京市自然科学基金项目(7212193) （7212193）
中国中医科学院科技创新工程项目(CI2021A02406) （CI2021A02406）
北京中医药薪火传承"新3+3"工程示范案例项目(2023-ZYSF-21) （2023-ZYSF-21）
