中华骨质疏松和骨矿盐疾病杂志2025，Vol.18Issue(5)：593-601,9.DOI:10.3969/j.issn.1674-2591.2025.05.009
成纤维细胞生长因子18对前成骨细胞增殖和分化的影响
Effect of fibroblast growth factor 18 on proliferation and differentiation of pre-osteoblasts
刘婷婷 1郭兴志 2陶蓓 3刘建民 3鲁平 4赵红燕3
作者信息
- 1. 200025 上海,上海交通大学医学院附属瑞金医院内分泌代谢病科||450000 郑州,阜外华中心血管病医院内分泌代谢病科
- 2. 200025 上海,上海交通大学医学院附属瑞金医院内分泌代谢病科||710068 西安,陕西省人民医院神经内科
- 3. 200025 上海,上海交通大学医学院附属瑞金医院内分泌代谢病科
- 4. 450000 郑州,阜外华中心血管病医院内分泌代谢病科
- 折叠
摘要
Abstract
Objective To investigate the effect of fibroblast growth factor 18(FGF18)on the proliferation and differentiation of pre-osteoblasts and the role of forkhead box protein O1(FOXO1)in this process in vitro.Methods CCK-8 assay was used to detect the effect of FGF18 on proliferation of MC3T3-E1 cells.MC3T3-E1 cells were cultured under mineralizing conditions with or without recombinant human FGF18 for 7 days and 21 days.Alkaline phos-phatase staining and alizarin red staining were performed to evaluate the differentiation and mineralization,respectively.Total RNA was isolated and q-PCR was performed to detect the expression of differentiation-related genes.After interfering with Fgf18-shRNA adenovirus,alkaline phosphatase staining was executed following the induction for seven days.Western blot was used to detect FOXO1 and its phosphorylation at different stages of differentiation.Results FGF18 promoted the proliferation while inhibited the differentiation and mineralization of pre-osteoblasts both in a dose-dependent way.After in-terfering with the Fgf18-shRNA adenovirus,osteoblast differentiation was significantly improved than the blank shRNA ad-enovirus control group.Protein electrophoresis analysis revealed that total FOXO1 had no considerable change,while p-FOXO1 was markedly up-regulated at the beginning of 0.5 h and 1 h of MC3T3-E1 osteogenic differentiation.However,this phenomenon was significantly inhibited after the addition of recombinant human FGF18.Conclusion FGF18 promotes the proliferation but inhibits the differentiation and mineralization of pre-osteoblasts in vitro in a dose-de-pendent way within limits.Combined with the results of previous studies it is speculated that FGF18 may have a stage-spe-cific role in promoting/inhibiting osteogenic differentiation.FGF18 inhibits p-FOXO1 level at the beginning of pre-osteo-blasts differentiation and may thus mediate the inhibition of pre-osteoblast differentiation through this pathway.关键词
成纤维细胞生长因子18/前成骨细胞/增殖/分化/叉头框蛋白O1Key words
fibroblast growth factor 18/pre-osteoblast/proliferation/differentiation/forkhead box protein O1分类
医药卫生引用本文复制引用
刘婷婷,郭兴志,陶蓓,刘建民,鲁平,赵红燕..成纤维细胞生长因子18对前成骨细胞增殖和分化的影响[J].中华骨质疏松和骨矿盐疾病杂志,2025,18(5):593-601,9.
