中成药2025，Vol.47Issue(11)：3533-3540,8.DOI:10.3969/j.issn.1001-1528.2025.11.001
UPLC-MS/MS法同时测定灯盏生脉胶囊中22种皂苷的含量
Simultaneous content determination of twenty-two saponins in Dengzhan Shengmai Capsules by UPLC-MS/MS
摘要
Abstract
AIM To establish a UPLC-MS/MS method for the simultaneouscontent determination of notoginsenosides R1,Fc,Fe,ginsenosides Re,Rg1,Rf,F3,Rg2,Ra2,Rb1,Ro,Rc,F1,Ra1,Rb2,Rb3,Rd,F2,Rg5,chikusetsusaponin Ⅳ a,20(S)-ginsenoside Rg3 and 20(R)-ginsenoside Rg3 in Dengzhan Shengmai Capsules.METHODS The analysis was performed on a 35 ℃ thermostatic Accucore Phenyl Hexyl column(2.1 mm×100 mm,2.6 μm),with the mobile phase comprising of 0.1％formic acid-acetonitrile flowing at 0.4 mL/min in a gradient elution manner,and heated electrospray ionization source was adopted in negative ion scanning with parallel reaction monitoring mode.Subsequently,cluster analysis,principal component analysis and partial least squares discriminant analysis were adopted.RESULTS Twenty-two saponins showed good linear relationships within their own ranges(r ≥ 0.996 4),whose average recoveries were 98.5％-101.6％with the RSDs of 1.3％-5.2％.Ten batches of samples were clustered into four types,three principal components demonstrated the accumulative variance contribution rate of 90.265％,ginsenosides Rb1,Rg2,Rb2,Rd,Rg1,Ro,Rf,Re,Rg5,Rc were taken as potential quality markers.CONCLUSION This simple,efficient,accurate and sensitive method can be used for the quality control of Dengzhan Shengmai Capsules.关键词
灯盏生脉胶囊/皂苷/含量测定/UPLC-MS/MS/聚类分析/主成分分析/偏最小二乘判别分析Key words
Dengzhan Shengmai Capsules/saponins/content determination/UPLC-MS/MS/cluster analysis/principal component analysis/partial least squares discriminant analysis分类
医药卫生引用本文复制引用
庄珊珊,周琳,洪芳,林隆,林思荣,黄鸣清..UPLC-MS/MS法同时测定灯盏生脉胶囊中22种皂苷的含量[J].中成药,2025,47(11):3533-3540,8.基金项目
国家自然科学基金面上项目(82274080) （82274080）
