中成药2025，Vol.47Issue(11)：3548-3554,7.DOI:10.3969/j.issn.1001-1528.2025.11.003
基于HPLC指纹图谱及含量测定完善复方鱼腥草片质量
Quality improvement of Compound Yuxingcao Tablets based on HPLC fingerprints and content determination
摘要
Abstract
AIM To improve the quality of Compound Yuxingcao Tablets.METHODS The HPLC fingerprints were established,after which the contents of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,forsythoside I,forsythoside A,quercitrin,3,5-O-dicaffeoylquinic acid,4,5-O-dicaffeoylquinic acid,baicalin,oroxyloside,wogonin and baicalein were determined.The analysis was performed on a 30 ℃ thermostatic Waters XBridge C18 column(250 mm×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1％phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 327 nm.Subsequently,principal component analysis and orthogonal partial least squares discriminant analysis were adopted.RESULTS There were 21 common peaks in the fingerprints for 22 batches of samples with the similarities of more than 0.85.Twelve constituents showed good linear relationships within their own ranges(R20.999 8),whose average recoveries were 93.68％-101.16％with the RSDs of 0.95％-2.35％.Baicalin,wogonin and forsythoside A were taken as quality differential components.CONCLUSION This simple and accurate method can be used for the quality control of Compound Yuxingcao Tablets.关键词
复方鱼腥草片/质量完善/HPLC指纹图谱/含量测定/主成分分析/正交偏最小二乘法判别分析Key words
Compound Yuxingcao Tablets/quality improvement/HPLC fingerprints/content determination/principal component analysis/orthogonal partial least squares discriminant analysis分类
医药卫生引用本文复制引用
艾光丽,王欣,李及,李婷婷,罗霄,马双成..基于HPLC指纹图谱及含量测定完善复方鱼腥草片质量[J].中成药,2025,47(11):3548-3554,7.基金项目
国家重点研发计划-中医药现代化专项(2023YFC3504100) （2023YFC3504100）
