中成药2025，Vol.47Issue(11)：3580-3585,6.DOI:10.3969/j.issn.1001-1528.2025.11.007
白花丹总黄酮纤维素酶辅助超声提取工艺优化及其抗氧化活性评价
Optimization of cellulase-assisted ultrasound extraction process for total flavonoids from Plumbago zeylanica and evaluation of their anti-oxidant activity
摘要
Abstract
AIM To optimize the cellulase-assisted ultrasound extraction process for total flavonoids from Plumbago zeylanica L.,and to evaluate their anti-oxidant activity.METHODS With extraction time,liquid-solid ratio,cellulase addition amount,extraction temperature and ultrasonic power as influencing factors,extraction rate of total flavonoids as an evaluation index,the extraction process was optimized by response surface method on the basis of single factor test.Subsequently,The scavenging rates of extract on DPPH,ABTS and OH free radicals were determined.RESULTS The optimal conditions were determined to be 34∶1 for liquid-solid ratio,3％for cellulase addition amount,51 ℃ for extraction temperature,38 min for extraction time,and 400 W for ultrasonic power,the extraction rate of total flavonoids was(33.411±0.97)％.The IC50 values of three free radicals were 0.13,0.042,3.29 mg/mL,respectively.CONCLUSION This reasonable and reliable method can be used for the cellulase-assisted ultrasound extraction of total flavonoids from P.zeylanica with strong anti-oxidant activity.关键词
白花丹/总黄酮/纤维素酶/超声提取工艺/抗氧化活性/响应面法Key words
Plumbago zeylanica L./total flavonoids/cellulase/ultrasound extraction process/anti-oxidant activity/response surface method分类
医药卫生引用本文复制引用
高小露,程文德,文月元,幸尚平,施承,朱丹,许亚楠..白花丹总黄酮纤维素酶辅助超声提取工艺优化及其抗氧化活性评价[J].中成药,2025,47(11):3580-3585,6.基金项目
广西自然科学基金项目(2023GXNSFBA026208,2025GXNSFAA069843) （2023GXNSFBA026208,2025GXNSFAA069843）
大学生创新创业训练项目(S202310598115,S202410598142,S202410598148) （S202310598115,S202410598142,S202410598148）
广西科技重大专项(桂科 AA22096021) （桂科 AA22096021）
广西四新研究与实践项目(XNK202414) （XNK202414）
