种子2025，Vol.44Issue(10)：11-20,36,11.DOI:10.16590/j.cnki.1001-4705.2025.10.011
玉米生长素应答因子ARF基因家族的全基因组鉴定及表达特征分析
Genome-wide Identification and Expression Profile Analysis of the Auxin Response Factor ARF Gene Family in Maize
摘要
Abstract
Drought stress is one of the key abiotic adverse factors restricting maize(Zea mays L.)pro-duction.In order to explore the physiological functions and mechanisms of action of maize auxin response factor(ARF)in the process of responding to drought stress,maize variety Zheng8713 was used as the experimental materialBased on whole-genome analysis,the members of the ARF gene family in maize were identified and analyzed by bioinformatics,and the expression patterns of related genes under drought stress conditions were analyzed by RT-qPCR technology.The results indicated that a total of 28 ARF genes were identified in the maize genome.Physicochemical property analysis showed that the amino acid number of ARF protein was from 511 to 1 152,and the isoelectric point range was 5.70 to 8.08.All of them were hydrophilic and unstable proteins.The subcellular localiza-tion prediction results indicated that all 28 members of the ZmARF family were located within the nucleus.Phylogenetic analysis revealed that the ARF family members of maize could be classified into five subfamilies,and most of their genes had a high degree of homology with those of Sorghum bicol-or and Oryza sativa.Conserved domain analysis indicated that all ARF proteins contained the Auxin_resp and B3 conserved domains,and their promoter regions were rich in abolic acid response elements and methyl jasmonate response elements.Tissue-specific expression analysis indicated that all 28 ZmARF genes were expressed in the tested tissues,and the expression patterns of genes in the same subfamily were relatively similar.Analysis of expression characteristics under drought stress re-vealed that the expression levels of most ZmARFs significantly increased or decreased when exposed to drought stress.关键词
玉米/生长素响应因子/生物信息学/表达模式Key words
Zea mays L./auxin response factors/bioinformatics/expression pattern分类
农业科技引用本文复制引用
马晨晨,叶飞宇,张晓东,胡德升,田艳雨,孙文鑫,李果,邓亚洲,曹丽茹..玉米生长素应答因子ARF基因家族的全基因组鉴定及表达特征分析[J].种子,2025,44(10):11-20,36,11.基金项目
河南省重点研发项目(251111111500) （251111111500）
神农种业实验室一流课题(SN01-2022-02) （SN01-2022-02）
