种子2025，Vol.44Issue(10)：21-27,53,8.DOI:10.16590/j.cnki.1001-4705.2025.10.021
基于简化基因组测序的巨桉引种林群体遗传分析
Population Genetic Analysis of Eucalyptus grandis Introduction Forests Based on Reduced-representation Genome Sequencing
摘要
Abstract
Eucalyptus grandis is an economic tree widely planted in southern regions.In order to pro-mote the breeding process of Eucalyptus grandis,using the introduced Eucalyptus grandis forest in Dongmen Forest Farm of Guangxi as the material,through second-generation sequencing technology,simplified genome sequencing was conducted on the first batch of 78 introduced and preserved Euca-lyptus grandis plants to develop a batch of highly specific single nucleotide polymorphism markers and analyze the genetic structure and genetic diversity of the existing population.The results showed that a total of 10 258 918 SNP loci were obtained.By using bioinformatics analysis methods,the genetic analysis of the population of Eucalyptus grandis was completed.Through phylogenetic tree analysis,Eucalyptus grandis was classified into four major categories,and the genetic diversity parameters such as the average observed heterozygosity,nucleotide diversity and average inbreeding number of each classification of Eucalyptus grandis were calculated.Population structure and princi-pal component analysis further demonstrated that the plants in the introduced Eucalyptus grandis forests had a relatively distant genetic relationship and significant differences in genetic diversity,thus possessing a high value for genetic resource preservation.关键词
巨桉/简化基因组测序/SNP/群体遗传分析Key words
Eucalyptus grandis/reduced representation genome sequencing/SNP/population genetic analysis分类
生物科学引用本文复制引用
邓紫宇,张磊,周韬,熊涛,胡旭,陈升侃,王建忠..基于简化基因组测序的巨桉引种林群体遗传分析[J].种子,2025,44(10):21-27,53,8.基金项目
广西揭榜制科技项目(桂科 JB22035001) （桂科 JB22035001）
广西科技基地和人才专项(桂科 AD24010046、桂科 AD23049002) （桂科 AD24010046、桂科 AD23049002）
