种子2025，Vol.44Issue(10)：37-43,86,8.DOI:10.16590/j.cnki.1001-4705.2025.10.037
金银花抗旱性评价及转录组分析
Evaluation on of Drought Resistance of Lonicera japonica Thunb. and Transcriptome Analysis
摘要
Abstract
Lonicera japonica,as a traditional Chinese medicinal herb,had the effect of clearing heat and detoxifying.However,during its growth and development,it is highly susceptible to external biological and abiotic stresses.To explore the physiological and molecular response mechanisms of Lonicera japonica under drought stress conditions,25％PEG-6000 was used to simulate drought stress,and Fenghua No.3 was subjected to drought treatment.Transcriptome sequencing was utilized to discover key differentially expressed genes.The results showed that after drought simulation treat-ment,the contents of malondialdehyde(MDA),proline(Pro),and the activities of superoxide dis-mutase(SOD)and catalase(CAT)in honeysuckle all increased to varying degrees.Transcriptome analysis including multiple metabolic pathways such as plant hormone signal transduction,phenylpro-panoid biosynthesis,photosynthesis and alpha-linolenic acid metabolism,as well as multiple differen-tial transcription factors including LjRHL4,LjPIF4,LjERF14,LjMYB14 and LjNAC27,which were involved in the process of Lonicera japonica resistance to drought stress.关键词
金银花/干旱胁迫/转录组/生理机制Key words
Lonicera japonica Thunb./drought stress/transcriptome/physiological mechanism分类
农业科技引用本文复制引用
张晓申,吴强,曹辉,左红娟,王广军,佟斌,王慧瑜..金银花抗旱性评价及转录组分析[J].种子,2025,44(10):37-43,86,8.基金项目
国家现代农业产业技术体系(CARS-21) （CARS-21）
河南省林木科技兴林项目"金银花优质安全高效栽培技术集成与应用"(豫财环资[2023]60号) （豫财环资[2023]60号）
河南省林木科技兴林项目"金银花新品种选育及提质增效栽培技术研究与示范"(豫财环资[2025]44号) （豫财环资[2025]44号）
