种子2025，Vol.44Issue(10)：60-68,9.DOI:10.16590/j.cnki.1001-4705.2025.10.060
干旱胁迫对过表达羽衣甘蓝BoNAC02基因烟草种子萌发的影响
The Effect of Drought Stress on Seed Germination of Nicotiana tabacum Overexpressing the Brassica oleracea var. acephala of BoNAC02 Gene
摘要
Abstract
The NAC transcription factor family plays a significant role in plant growth,defense regula-tion and stress response.However,in kale,the drought resistance mechanism of NAC has not yet been described.In this study,the BoNAC02 gene from Brassica oleracea var.acephala was cloned,its bioinformatics was analysed,and induced its expression with mannitol and abscisic acid(ABA)was induced.Overexpression of BoNAC02 in Nicotiana tabacum led to stronger drought tolerance in its seed germination.After drought treatment,the germination rate,germination potential and germi-nation index of the seeds were all higher than those of the wild type.Transgenic seedlings had rela-tively high activities of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT),as well as glutathione S transferase(GST)and ascorbic acid peroxidase(APX),but the content of malondial-dehyde(MDA)was relatively low.In addition,several genes related to the ABA signaling pathway,DREB1A and reactive oxygen species scavenging system were significantly upregulated in transgenic lines.关键词
BoNAC02基因/干旱胁迫/转基因烟草/种子萌发Key words
BoNAC02 gene/drought stress/transgenic tobacco/seed germination分类
农业科技引用本文复制引用
杨勇,杨伟民,陶静,冷鹭婷,邵伟伦,蒋炳伸,李鸿雁,李大红..干旱胁迫对过表达羽衣甘蓝BoNAC02基因烟草种子萌发的影响[J].种子,2025,44(10):60-68,9.基金项目
河南省科技攻关项目"旱地酸性农田地力提升的关键技术研究与应用"(252102110204) （252102110204）
