针灸临床杂志2025，Vol.41Issue(11)：53-59,7.DOI:10.19917/j.cnki.1005-0779.025217
基于PI3K/Akt/FoxO3a信号通路探讨针刀改善制动诱导大鼠腓肠肌萎缩的作用机制
Mechanistic Study of Acupotomy in Ameliorating Immobilization-Induced Rat Gastrocnemius Muscle Atrophy via PI3K/Akt/FoxO3a Signaling Pathway
摘要
Abstract
Objective:To investigate the mechanism by which acupotomy intervenes in immobilization-induced gastrocnemius muscle atrophy in rats via the PI3K/Akt/FoxO3a signaling pathway.Methods:32 male Wistar rats were randomly assigned to the blank control,the model group,the stretching group,and the acupotomy group.The immobilization-induced gastrocnemius muscle atrophy model was established by fixing the right hindlimb ankle joint in plantar flexion for 4 weeks.After successful modeling,the stretching group received a 0.3 N force applied along the dorsiflexion direction of the right ankle,perpendicular to the plantar surface.In the acupotomy group,a 0.4 mm ×25 mm acupotomy needle was used to release the fascia on the muscle surface,followed by the same stretching method as the stretching group.Following the intervention,ankle dorsiflexion range of motion(ROM)was measured,and gastrocnemius muscle strength,wet weight,and length were assessed.RT-PCR was used to measure mRNA expressions of PI3K,Akt,FoxO3a,MyoD1,and Pax7 in the gastrocnemius,while Western blot was performed to detect the protein expressions of PI3K,Akt,p-PI3K,and p-Akt.Results:In the acupotomy group,ankle dorsiflexion ROM,gastrocnemius muscle strength,wet weight,and length,mRNA expressions of PI3K,Akt,MyoD1,and Pax7,as well as protein expressions of p-PI3K and p-Akt were all significantly increased(P＜0.05,P＜0.01),and FoxO3a mRNA expression was significantly decreased compared with the model group and stretching group after the treatment(P＜0.01).Conclusion:Acupotomy can effectively delay gastrocnemius muscle atrophy in rats,and its mechanism may be closely related to the PI3K/Akt/FoxO3a signaling pathway.关键词
肌萎缩/腓肠肌/针刀/制动诱导/PI3K/Akt/FoxO3a/MyoD1/Pax7Key words
Muscle atrophy/Gastrocnemius/Acupotomy/Immobilization-induction/PI3K/Akt/FoxO3a/MyoD1/Pax7分类
医药卫生引用本文复制引用
李宛容,张晗,贾文睿,卢美璇,张义..基于PI3K/Akt/FoxO3a信号通路探讨针刀改善制动诱导大鼠腓肠肌萎缩的作用机制[J].针灸临床杂志,2025,41(11):53-59,7.基金项目
河北省中医药管理局科研计划,编号:B2025021. （）
