针灸临床杂志2025，Vol.41Issue(11)：68-73,6.DOI:10.19917/j.cnki.1005-0779.025219
针刀干预对膝骨关节炎兔软骨细胞TFR1、FPN蛋白表达的影响
Effects of Acupotomy Intervention on Protein Expressions of TFR1 and FPN in Chondrocytes of KOA Rabbits
摘要
Abstract
Objective:To investigate the effects of acupotomy on iron ion metabolism in chondrocytes of rabbits with knee osteoarthritis(KOA),and to explore its underlying therapeutic mechanisms.Methods:A total of 24 male New Zealand rabbits were randomly assigned into the blank control group,the model group,the acupotomy group,and the electro-acupuncture(EA)group.The KOA model was established using a 6-week immobilization protocol in an overextension position(modified Videman method).Treatment interventions for the acupotomy group and the EA group lasted 3 weeks.Behavioral assessments were performed using the Lequesne MG index and passive range of motion(PROM)of the knee joint.Histological morphology of chondrocytes was examined with HE staining,and mitochondrial ultrastructure was observed via transmission electron microscopy.Protein expressions of TFR1 and FPN in chondrocytes were determined using Western blot analysis.Results:Compared with the blank control group,rabbits in the model group exhibited significantly higher Lequesne MG scores and markedly reduced PROM of the knee joint(P＜0.01);chondrocyte arrangement was disorganized,inflammatory cell infiltration was prominent,and mitochondrial changes included shrinkage,reduced cristae,or even disappearance;the TFR1 expression was significantly up-regulated,whereas the FPN expression was markedly suppressed in the model group(P＜0.01).Compared with the model group,both the acupotomy group and the EA group showed significantly lower Lequesne MG scores and significantly higher PROM values(P＜0.01);chondrocyte arrangement was more regular,inflammatory cell infiltration was reduced,and mitochondria were more numerous with relatively normal structure and increased cristae;the protein expression of TFR1 was significantly down-regulated(P＜0.01,P＜0.05),whereas the protein expression of FPN was markedly up-regulated in the acupotomy group and the EA group(P＜0.01).Compared with the EA group,the acupotomy group showed a greater reduction in Lequesne MG scores(P＜0.05)and a more pronounced increase in PROM(P＜0.01);in addition,TFR1 protein expression was lower(P＜0.05)and FPN protein expression was significantly higher(P＜0.01)in the acupotomy group.Conclusion:Acupotomy intervention can regulate the protein expressions of TFR1 and FPN and enhance iron ion metabolism,thereby protecting articular cartilage.Its therapeutic efficacy is superior to that of EA.关键词
膝骨关节炎/针刀/铁离子代谢/铁死亡Key words
Knee osteoarthritis/Acupotomy/Iron ion metabolism/Ferroptosis分类
医药卫生引用本文复制引用
崔永奇,史晓伟,马芸瑄,齐昕,马薇薇,郭长青..针刀干预对膝骨关节炎兔软骨细胞TFR1、FPN蛋白表达的影响[J].针灸临床杂志,2025,41(11):68-73,6.基金项目
国家自然科学基金面上项目,编号:82074523. （）
