作物学报2025，Vol.51Issue(12)：3133-3143,11.DOI:10.3724/SP.J.1006.2025.52012
抗白叶枯病广亲和恢复系的创制及杂交育种应用研究
Development of wide-compatibility restorer lines resistant to bacterial blight and their application in rice hybrid breeding
摘要
Abstract
Zhenghui 084(Xa7),IRBB21(Xa21),CBB23(Xa23),and IRBB27(Xa27)were used as donor parents to develop gene-specific functional molecular markers and introduce target resistance genes into the wide-compatibility restorer line F7540 through gene pyramiding.The resulting improved restorer lines,along with recurrent parents,were inoculated with nine races of Xanthomonas oryzae pv.oryzae(Xoo).Six improved resistant restorer lines were then crossed with three indica male sterile lines(Tai 1S,Quan 9311A,Huazhe 2A)and three japonica male sterile lines(Chunjiang 16A,Chunjiang 88A,Huazhong 2A).Both hybrid combinations and parental lines were inoculated with two representative Xoo races,Zhe173 and PXO99,and agronomic traits were also evaluated.The results showed that nine restorer lines carrying various resistance genes were successfully devel-oped through multi-generation selection.Among them,F7540-Xa7-Xa23-Xa27 exhibited high resistance to all nine Xoo races.F7540-Xa7-Xa23 and F7540-Xa23 also showed resistance or high resistance to all nine races.Lines F7540-Xa7-Xa21 and F7540-Xa7-Xa21-Xa27 were resistant to eight races,while F7540-Xa7 and F7540-Xa21 were resistant to seven and two races,respectively.Notably,F7540-Xa27 showed no resistance to any of the tested races.In the hybrid inoculation tests with Zhe173 and PXO99,all combinations involving F7540-Xa23 and F7540-Xa7-Xa23 showed disease resistance.Hybrids derived from F7540-Xa7-Xa21 crossed with japonica male sterile lines also exhibited resistance.Additionally,the combination of F7540-Xa21 and Quan 9311A was resistant to disease.Comprehensive evaluation of resistance and agronomic performance indicated that the hybrid combinations of F7540-Xa23 with Chunjiang 88A and Quan 9311A possessed excellent disease resistance and favorable agronomic traits,demonstrating high breeding potential.关键词
抗性基因/白叶枯病/基因聚合/广亲和恢复系/杂交育种Key words
resistance genes/bacterial blight/gene pyramiding/wide-compatibility restorer line/hybrid breeding引用本文复制引用
谢留杰,段敏,杨勇,潘晓飚,马伯军,黄善军,陈析丰..抗白叶枯病广亲和恢复系的创制及杂交育种应用研究[J].作物学报,2025,51(12):3133-3143,11.基金项目
本研究由浙江省基础公益研究计划项目(LTGN24C130002,LQ24C060003)和浙江省农业新品种选育重大科技专项(2021C02063-5-5)资助.This study was supported by the Basic Public Welfare Research Project of Zhejiang Province(LTGN24C130002,LQ24C060003)and the Zhejiang Province Major Science and Technology Special Project for the Breeding of New Agricultural Varieties(2021C02063-5-5). （LTGN24C130002,LQ24C060003）
