作物学报2025，Vol.51Issue(12)：3157-3170,14.DOI:10.3724/SP.J.1006.2025.52020
基于CSSL-Z267的单/双片段代换系的水稻产量性状QTL遗传效应解析与设计育种应用
Genetic dissection and breeding application of rice yield-related QTL using single and dual segment substitution lines derived from CSSL-Z267
摘要
Abstract
Rice yield-related traits,as typical quantitative traits,are controlled by multiple genes with minor effects.Mapping these genes using single segment substitution lines(SSSLs)not only provides an ideal system for dissecting their molecular mechanisms,but also lays a critical foundation for whole-genome design breeding by minimizing interference from genetic back-ground.In this study,the chromosome segment substitution line Z267—carrying five donor segments in a Nipponbare genetic background—was used to construct a Nipponbare/Z267 F2 population,through which nine yield-related QTL were successfully identified.Further genetic dissection yielded five SSSLs and one dual segment substitution line(DSSL).The results showed that all five SSSLs(S1-S5)carried positive-effect QTL that significantly increased grain length and secondary branch number,while also harboring negative-effect QTL that reduced grain width.In the DSSL(D1),multiple QTL interactions were observed:com-binations of panicle length loci(qPL6 with qPL1),primary branch number loci(qNPB6 with qNPB1),and secondary branch number loci(qNSB1 with qNSB6)exhibited positively transgressive inheritance effects.Meanwhile,combinations of grain width(qGW6 with qGW1)and 1000-grain weight(qGWT6 with qGWT1)loci displayed negatively transgressive inheritance.The ge-netic effects of grain length(qGL6 with qGL1)and plant height(qPH6 with qPH1)locus combinations were comparable to those of the single locus qGL1 and qPH6,respectively.Genetic effect analysis indicated that hybrid combinations of S1 and S5 could be effectively used to develop elite lines with taller plant architecture and slender grain morphology.Overall,this study systemati-cally dissected the genetic effects of yield-related QTL,providing valuable theoretical insights and germplasm resources for elu-cidating molecular mechanisms and advancing whole-genome design breeding in rice.关键词
水稻/产量性状/QTL/染色体片段代换系/加性效应/QTL互作Key words
rice/yield traits/QTL/chromosome segment substitution line/additive effect/QTL interaction引用本文复制引用
张瀚,余金琎,谭林璐,张婧泉,王小董,谢庄,谢可盈,凌英华,赵芳明..基于CSSL-Z267的单/双片段代换系的水稻产量性状QTL遗传效应解析与设计育种应用[J].作物学报,2025,51(12):3157-3170,14.基金项目
本研究由国家自然科学联合基金项目(U23A20184)和重庆市自然科学基金面上项目(CSTB2023NSCQ-MSX0124)资助. This study was supported by the National Natural Science Union Foundation of China(U23A20184)and the Chongqing Natural Science Foundation Project(CSTB2023NSCQ-MSX0124). （U23A20184）
