作物学报2025，Vol.51Issue(12)：3171-3183,13.DOI:10.3724/SP.J.1006.2025.53024
基于抗旱玉米自交系SL001的抗旱优异基因资源挖掘
Identifying of excellent drought-tolerant gene resources based on drought-tolerant maize inbred line SL001
摘要
Abstract
Drought is one of the most severe abiotic stresses limiting the growth and development of maize.Identifying drought-resistant genes and applying them to the development of new drought-tolerant varieties is an effective strategy to address this challenge.In this study,the drought-sensitive inbred line B73 and the drought-tolerant inbred line SL001 were used to evalu-ate drought tolerance phenotypes.SL001 exhibited a lower degree of wilting and a significantly higher survival rate after rehydra-tion compared to B73.In addition,under drought conditions,SL001 showed significantly higher relative water content and net photosynthetic rate than B73.Transcriptome analysis of B73 and SL001 under varying drought stress conditions identified a total of 11,240 differentially expressed genes(DEGs),of which 4354 were specifically expressed under moderate and severe drought stress,but not under well-watered conditions.These DEGs were mainly enriched in plant hormone signaling and plant-pathogen interaction pathways.Among them,two candidate drought-resistance genes,Zm00001eb439810 and Zm00001eb365420,were predicted and further validated by qRT-PCR.The results suggested that Zm00001eb439810 may positively regulate the maize drought stress response,whereas Zm00001eb365420 may act as a negative regulator.This study provides valuable genetic resources and potential targets for improving drought tolerance in maize.关键词
玉米/干旱胁迫/转录组分析/脱落酸/表达量Key words
maize/drought stress/transcriptome analysis/ABA/expression level引用本文复制引用
魏琦,路运才,李春辉,何冠华,张登峰,李永祥,刘旭洋,唐怀君,刘成,王天宇,黎裕..基于抗旱玉米自交系SL001的抗旱优异基因资源挖掘[J].作物学报,2025,51(12):3171-3183,13.基金项目
本研究由国家自然科学基金项目(32201751),财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-02-03)和黑龙江省高等学校基本科研业务费项目(2024-KYYWF-0119)资助. This study was supported by the National Natural Science Foundation of China(32201751),the China Agriculture Research System of MOF and MARA(CARS-02-03),and the Basic Research Funds for Higher Education Institutions in Heilongjiang Province(2024-KYYWF-0119). （32201751）
