作物学报2025，Vol.51Issue(12)：3184-3197,14.DOI:10.3724/SP.J.1006.2025.55032
过表达BnNRT2.3-like对油菜氮素吸收利用及产量的影响
Effects of BnNRT2.3-like overexpression on nitrogen uptake,utilization effi-ciency,and yield in Brassica napus L
摘要
Abstract
Nitrate transporter 2(NRT2)is a high-affinity nitrate transporter that plays a crucial role in nitrate uptake and translo-cation in plants.In this study,we identified a key low-nitrogen-responsive gene,BnNRT2.3-like,using real-time quantitative PCR(RT-qPCR).Bioinformatics analysis was conducted,and an overexpression vector of BnNRT2.3-like was constructed to generate transgenic plants.Key agronomic traits of rapeseed at the mature stage were evaluated through a natural pot experiment.At the seedling stage,we assessed nitrogen accumulation,nitrogen use efficiency(NUE),chlorophyll content,expression levels of ni-trogen utilization-related genes,and the activities of nitrate reductase(NR)and glutamine synthetase(GS).The results indicated that BnNRT2.3-like protein was found to have a molecular weight of 61.87 kD and an isoelectric point of 9.08,exhibiting typical features of the MFS superfamily and NNP gene family.Compared to the wild type(WT),BnNRT2.3-like overexpression lines showed increases of 9.1%in plant height,20.0%in thousand-seed weight,and 62.1%in grain yield per plant.Significant improvements were also observed in effective branch height,main inflorescence length,number of siliques per main inflorescence,total siliques per plant,silique length,and average seeds per silique.Under low-nitrogen(LN)conditions,the overexpression lines exhibited an average increase of 1.22 cm in primary root length,a 0.633 g increase in fresh weight,and 55.0%and 13.6%in-creases in shoot and root dry weights,respectively,resulting in a 42.1%increase in total dry weight per plant.Shoot nitrogen ac-cumulation was also 17.0%higher than in WT.Furthermore,under LN stress at the seedling stage,the overexpression lines dem-onstrated significant enhancements in nitrogen accumulation,NUE,NR and GS activities,and chlorophyll content compared to WT plants.Notably,expression levels of nitrogen utilization-related genes(BnNPF4.6,BnNPF6.3-like,and BnAMT1.1a)were upregulated in the leaves but markedly downregulated in the roots.Together,these findings demonstrate that BnNRT2.3-like overexpression enhances low-nitrogen tolerance and yield-related traits in rapeseed,providing valuable germplasm resources and a theoretical foundation for improving nitrogen use efficiency in Brassica napus.关键词
甘蓝型油菜/BnNRT2.3-like/硝酸盐/氮素利用率/产量Key words
Brassica napus L./BnNRT2.3-like/nitrate/nitrogen use efficiency/yield引用本文复制引用
黄绒,周渠晨,陈楚铭,罗倩,易东,杜常欢,黄祥宇,盛锋,杜雪竹..过表达BnNRT2.3-like对油菜氮素吸收利用及产量的影响[J].作物学报,2025,51(12):3184-3197,14.基金项目
本研究由湖北省科技创新计划项目(2025BBB012)和科技创新2030—重大项目(2023ZD04070)资助. This study was supported by the Hubei Provincial Science and Technology Innovation Plan Project(2025BBB012)and the Science and Technology Innovation 2030-Major Project(2023ZD04070). （2025BBB012）
