作物学报2025，Vol.51Issue(12)：3198-3210,13.DOI:10.3724/SP.J.1006.2025.54052
薏苡VQ4基因的克隆及耐盐性初步分析
Cloning of the VQ4 gene and preliminary analysis of its role in salt tolerance in Coix lacryma-jobi L
摘要
Abstract
VQ proteins,a plant-specific protein family characterized by a conserved VQ domain,have garnered considerable at-tention due to their critical roles in abiotic stress responses as well as plant growth and development.In this study,the Coix lacryma-jobi L.cultivar'Wanyi 2'was used as the experimental material.A combination of bioinformatics analysis,quantitative real-time PCR,subcellular localization,and yeast two-hybrid assays was employed to characterize the molecular properties of ClVQ4.Its function in salt stress response was further investigated through heterologous expression in yeast and genetic trans-formation in Arabidopsis thaliana.The results revealed that ClVQ4 contains an open reading frame(ORF)of 594 bp,encoding a 197-amino-acid protein predicted to be an unstable,hydrophilic protein with an isoelectric point(pI)of 6.43.Cis-acting element analysis of the ClVQ4 promoter identified multiple hormone-and stress-responsive elements.The expression of ClVQ4 was in-duced by methyl jasmonate(MeJA)and abscisic acid(ABA),and significantly upregulated under salt stress.Subcellular localiza-tion and yeast two-hybrid assays showed that ClVQ4 is mainly localized in the nucleus and cell membrane,interacts with Coix WRKY30,and can form heterodimers with other VQ proteins.Under NaCl treatment,the yeast strain expressing ClVQ4 exhib-ited enhanced survival.Furthermore,overexpression of ClVQ4 in Arabidopsis resulted in higher germination and survival rates and longer root lengths compared to the wild type(WT)under salt stress.Additionally,transgenic lines exhibited significantly higher activities of antioxidant enzymes(POD,SOD,and CAT)and significantly lower malondialdehyde(MDA)content than WT,suggesting a positive regulatory role of ClVQ4 in salt stress tolerance.关键词
薏苡/ClVQ4/基因克隆/亚细胞定位/耐盐性Key words
Coix lacryma-jobi L./ClVQ4/gene cloning/subcellular localization/salt tolerance引用本文复制引用
王玉娇,王永乐,添长久,郁春旺,吕佳斌,朱加保..薏苡VQ4基因的克隆及耐盐性初步分析[J].作物学报,2025,51(12):3198-3210,13.基金项目
本研究由国家自然科学基金青年项目(3240151950)资助. This study was supported by the Young Scientists Fund of the National Natural Science Foundation of China(3240151950). （3240151950）
