作物学报2025，Vol.51Issue(12)：3211-3223,13.DOI:10.3724/SP.J.1006.2025.54045
不同耐荫性谷子对遮阴的生理响应及转录组分析
Physiological response and transcriptome analysis of foxtail millet with diffe-rent shading tolerances under shading stress
摘要
Abstract
To investigate the response mechanisms of foxtail millet cultivars with different shade tolerance to shading stress,Yugu 29(YG29)and Baogu 25(BG25)were used as experimental materials.Shading nets were applied during the grain filling stage to simulate shading conditions.We examined the differences between the two cultivars in yield traits,photosynthetic pigments,pho-tosynthetic parameters,antioxidant enzyme activities,and transcriptomic profiles.The results showed that shading stress signifi-cantly reduced yield,photosynthetic rate,transpiration rate,and stomatal conductance,while significantly increasing chlorophyll a and b contents as well as the activities of peroxidase(POD),superoxide dismutase(SOD),and catalase(CAT).Compared with BG25,YG29 exhibited higher yield,chlorophyll content,photosynthetic rate,and antioxidant enzyme activities,along with a lower chlorophyll a/b ratio,indicating greater tolerance to shading stress.Transcriptome analysis identified 2961 and 2966 differentially expressed genes(DEGs)in BG25 and YG29 under shading conditions,respectively.These DEGs were significantly enriched in 24 and 16 Gene Ontology(GO)categories and 13 and 6 Kyoto Encyclopedia of Genes and Genomes(KEGG)path-ways in BG25 and YG29,respectively.Four pathways were commonly enriched in both cultivars:photosynthesis-antenna pro-teins,porphyrin and chlorophyll metabolism,phenylpropanoid biosynthesis,and cyanoamino acid metabolism.Further analysis of key metabolic pathways revealed that,under shading stress,YG29 showed lower expression levels of chlorophyll degrada-tion-related genes(NOL,HCAR,SGR,PPH,and RCCR),and higher expression levels of genes related to carbon fixation(RBC,RCA,PEPC,MDH,GAPC,and FBA)and antioxidant enzymes(PRX,CAT)compared with BG25.These genes may play critical roles in the enhanced shade tolerance of YG29.关键词
谷子/遮阴胁迫/生理特性/产量/转录组Key words
foxtail millet/shading stress/physiological characteristics/yield/transcriptome引用本文复制引用
朱灿灿,李君霞,景雅,付森杰,秦娜,王春义,代书桃,魏昕,张程炀..不同耐荫性谷子对遮阴的生理响应及转录组分析[J].作物学报,2025,51(12):3211-3223,13.基金项目
本研究由河南省重点研发计划专项(231111110300,241111112100),财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-06),河南省产业技术体系专项(HARS-25-04),河南省农业科学院自主创新项目(2025ZC24)和河南省农业科学院创新团队专项(2024TD039)资助. This study was supported by the Key Research and Development Project of Henan Province(231111110300,241111112100),the China Ag-riculture Research System of MOF and MARA(CARS-06),the Henan Province Agriculture Research System(HARS-25-04),the Henan Academy of Agricultural Sciences Independent Innovation Fund Project(2025ZC24),and the Innovation Teams of Henan Academy of Agri-cultural Sciences(2024TD039). （231111110300,241111112100）
