作物学报2025，Vol.51Issue(12)：3224-3237,14.DOI:10.3724/SP.J.1006.2025.54063
马铃薯CEP基因家族鉴定及StCEP2小肽调控马铃薯不定根生长和耐盐性的功能分析
Genome-wide identification of CEP gene family in potato(Solanum tuberosum L.)and functional analysis of StCEP2 in regulating adventitious root growth and salt tolerance
摘要
Abstract
C-terminally encoded peptide(CEP)genes encode a class of secreted small peptides characterized by conserved struc-tural domains near the C-terminus.These genes form a multi-member family that plays crucial roles in regulating various physio-logical processes in plants,including root development,nitrogen uptake,and responses to environmental stresses.In this study,members of the CEP gene family in potato(Solanum tuberosum,StCEP)were identified and analyzed for their physicochemical properties,conserved motifs,phylogenetic relationships,and promoter cis-acting elements.Expression patterns of StCEP genes were examined across different potato tissues and under various environmental conditions using RNA-seq data.Additionally,the function of salt-induced StCEP genes was validated through exogenous application of synthetic StCEP peptides.A total of 11 StCEP genes were identified in the potato genome,distributed across four chromosomes.The encoded StCEP proteins contained between 1 and 9 CEP motifs,with variability in motif number among members.Phylogenetic analysis revealed that StCEP family members clustered with CEP genes from Arabidopsis thaliana and Solanum lycopersicum,forming two major clades comprising 21 and 26 members,respectively.Synteny analysis showed one syntenic gene pair between potato and A.thaliana,and 12 syntenic pairs between potato and S.lycopersicum.Promoter analysis indicated that StCEP genes are primarily regulated by light-responsive,hormone-responsive,developmental,and stress-related cis-elements.Expression profiling revealed that StCEP genes exhibit tissue-specific expression and are responsive to nitrogen availability,phytohormones(BAP,IAA,GA3,and ABA),and both biotic(e.g.,Phytophthora infestans,BABA,BTH)and abiotic(e.g.,NaCl)stress factors.Furthermore,exogenous appli-cation of the StCEP2 peptide promoted adventitious root formation and enhanced salt tolerance in potato plantlets under in vitro conditions.This study offers valuable insights into the functional roles of StCEP genes in regulating plant development and re-sponses to environmental stress,laying a foundation for future functional genomics and crop improvement research.关键词
马铃薯/CEP小肽/外施小肽/不定根生长/耐盐性Key words
potato/CEP peptide/exogenous peptide assay/adventitious root growth/salt-stress tolerance引用本文复制引用
张慧敏,刘柏林,宋银,党亚茹,桑佳男,焦文静,孙春怡,庄佳牧,魏玉树,张超,田鹏..马铃薯CEP基因家族鉴定及StCEP2小肽调控马铃薯不定根生长和耐盐性的功能分析[J].作物学报,2025,51(12):3224-3237,14.基金项目
本研究由国家重点研发计划项目(2022YFF1002700),陕西省重点研发计划项目(2021LLRH-07和2024NC-YBXM-002)和大学生创新创业训练计划项目(S202410712556)资助. This study was supported by the National Key R&D Program of China(2022YFF1002700),the Key R&D Program in Shaanxi Province(2021LLRH-07 and 2024NC-YBXM-002),and the Undergraduate Training Program for Innovation and Entrepreneurship(S202410712556). （2022YFF1002700）
