作物学报2025，Vol.51Issue(12)：3238-3250,13.DOI:10.3724/SP.J.1006.2025.51009
基于16K芯片的野生二粒小麦渗入系BAd7-209籽粒蛋白含量QTL定位
QTL mapping of grain protein content in the introgression line BAd7-209 derived from wild emmer
摘要
Abstract
The introgression line BAd7-209,characterized by a high grain protein content(GPC)despite lacking a functional NAM-B1 gene,has been officially named the wheat cultivar Triticum aestivum L.'Chuannong 1 Sicaomai'.It was developed from a cross between wild emmer(as the male parent)and the high-yielding but low-GPC common wheat variety Chuannong 16(CN16).In this study,BAd7-209 was used as the male parent and crossed with two low-GPC common wheat cultivars,Chuanyu 27(CY27)and CN16,to develop both a recombinant inbred line(RIL)F6:8 mapping population and an F6 validation population.The objective was to investigate the genetic basis of high GPC in BAd7-209 and to identify genetic resources for high-quality wheat breeding.Across four environments over two consecutive years,BAd7-209 exhibited a mean GPC of 15.35%,significantly higher than that of the female parent CY27(12.30%).However,its thousand-grain weight(TGW)was 46.08 g—significantly lower than CY27(50.53 g).The RIL population showed a mean GPC of 14.01%and a TGW of 52.02 g,both significantly higher than those of the parent lines.This indicates that the high GPC from BAd7-209 and the high TGW from CY27 were effectively transferred to the RIL population,minimizing the influence of the concentration effect and enabling more accurate evaluation of GPC.A total of five quantitative trait loci(QTLs)associated with GPC were identified,explaining 9.68%to 29.17%of the phe-notypic variation.Notably,a novel major QTL,QGPC.sicau-YZ-6BS,derived from BAd7-209,was consistently associated with high GPC and accounted for 9.68%-29.17%of the variation.A functional KASP marker linked to this QTL was developed.Lines carrying this favorable locus exhibited significantly higher GPC than those without it(p<0.01)across all tested environments and genetic backgrounds,with GPC increases of 17.89%and 41.20%in the mapping and validation populations,respectively.Addi-tionally,thirteen novel wheat germplasm lines with superior traits—including grain quality,agronomic performance,and disease resistance—were selected from the 104 RILs.Their GPCs ranged from 13.99%to 18.08%,and TGWs ranged from 45.20 g to 57.69 g.These findings provide valuable insights for future genetic research and the breeding of high-yield,high-quality wheat cultivars.关键词
小麦/野生二粒小麦/渗入系/籽粒蛋白含量/QTL定位/优质高产/遗传育种Key words
common wheat/wild emmer/introgression line/grain protein content/QTL mapping/high quality and yield/genetic and breeding引用本文复制引用
王哲,郑有良,刘登才,黄林,伍碧华,胡燕灵,龚方仪,易睿,赵书宏,刘睿琴,刘雨杭,张甜,张亚洲..基于16K芯片的野生二粒小麦渗入系BAd7-209籽粒蛋白含量QTL定位[J].作物学报,2025,51(12):3238-3250,13.基金项目
本研究由四川省国际科技创新合作项目(2021YFH0110)和四川省科学技术厅重点研发计划项目(2021YFY2002-3-3)资助. This study was supported by the Sichuan Provincial International Science and Technology Innovation Cooperation Project(2021YFH0110)and Key R&D Project of Sichuan Provincial Department of Science and Technology(2021YFY2002-3-3). （2021YFH0110）
