植物学报2025，Vol.60Issue(6)：863-874,12.DOI:10.11983/CBB24156
小麦14-3-3蛋白TaGRF3-D基因克隆及功能分析
Cloning and Functional Analysis of the 14-3-3 Protein-encoding Gene TaGRF3-D in Wheat(Triticum aestivum)
摘要
Abstract
INTRODUCTION:14-3-3 proteins are a highly conserved protein family that specifically recognize phosphorylated target proteins and play crucial roles in plant abiotic stress responses.By interacting with AREB/ABF(ABA-responsive element binding protein/ABA-responsive element binding factor)transcription factors,14-3-3 proteins participate in ABA signal transduction and regulate abiotic stress tolerance.TaGRF3-D is a 14-3-3 protein gene in wheat(Triticum aestivum),and our previous studies revealed that the expression of this gene was upregulated under ABA and abiotic stress. RATIONALE:To explore the biological function of the TaGRF3-D gene,we cloned the gene,and investigated its subcel-lular localization and function under drought stress. RESULTS:The results revealed that TaGRF3-D is highly conserved in monocotyledonous plants and is localizes in the nucleus and plasma membrane.Compared with the wild type,the Arabidopsis thaliana transgenic lines overexpressing TaGRF3-D presented significantly longer roots under PEG and ABA treatments and showed a markedly greater survival rate after drought stress.Yeast two-hybrid analysis revealed that TaGRF3-D interacted with wheat TaABF3-B,TaABF4-A,TaABF15-D,TaABF16-B,TaABF17-D,and TaABF18-B,but not with TaABF1-D,TaABF2-AorTaABF19-A. CONCLUSION:These results suggest that TaABF3-D responds to ABA signaling by interacting with wheat TaABF tran-scription factors,thereby increasing the drought stress tolerance of transgenic plants.关键词
14-3-3蛋白/干旱胁迫/ABA响应/蛋白互作Key words
14-3-3 protein/drought stress/ABA response/protein interaction引用本文复制引用
孙月,郭树娟,赵惠贤,马猛,刘香利..小麦14-3-3蛋白TaGRF3-D基因克隆及功能分析[J].植物学报,2025,60(6):863-874,12.基金项目
陕西省自然科学基础研究计划(No.2024JC-YBMS-171)和国家自然科学基金(No.32072003,No.32372103) （No.2024JC-YBMS-171）
