植物学报2025，Vol.60Issue(6)：914-930,17.DOI:10.11983/CBB24191
不同钾水平下烟草根内皮层木栓化的适应性发育差异
Differences in the Adaptive Development of Suberin in the Tobacco Root Endothelial Layer under Different Potassium Levels
摘要
Abstract
INTRODUCTION:Apoplastic barriers differentiated from the root endoderm play important roles in plant stress resistance and nutrient uptake,and the development of suberin lamellae has become a popular research topic in recent years. RATIONALE:Hydroponic experiments with potassium concentrations ranging from 0.1 to 4.0 mmol·L-1 were conducted,using the cultivated tobacco variety Zhongyan 100 as the experimental material,to explore the effects of different potas-sium supplies on the endodermal suberization and its physiological and molecular mechanisms. RESULTS:Low potassium(0.1 mmol·L-1)stress significantly enhanced the endodermal suberization:the absolute length of the fully suberized region ranged from 0-2 cm in the control to 4-6 cm,and the relative proportion increased from 0-15.0％to 33.2％-44.3％.These findings indicate that suberization is one of the key morphological adaptation mechan-isms in tobacco under low potassium stress.Phenotypic analysis revealed that under low potassium stress,root elonga-tion increased while plant biomass decreased,and the potassium ion content and accumulation in both the aboveground and root parts decreased.Additionally,the flow rate and potassium ion concentration in the xylem sap decreased,indi-cating reduced transport efficiency.Endogenous hormone analysis revealed that low potassium stress increased the abscisic acid(ABA)content in roots while suppressing ethylene and methyl jasmonate levels,forming a specific hormonal regulatory network.The transcriptome data further supported the molecular basis of suberization development,showing significant upregulation of genes related to suberin synthesis and transport(e.g.,CYP86,GPAT,and ABCG)and their upstream positive regulatory factors(MYB36,MYB41,MYB92,and MYB93). CONCLUSION:This study is the first to reveal that low potassium stress regulates the suberization developmental pro-gram of tobacco roots through ABA-mediated hormonal signaling reprogramming,providing a novel perspective for un-derstanding the adaptation mechanisms of plants to potassium stress.关键词
木栓化/钾/根内皮层/激素Key words
suberization/potassium/root endodermis/hormone引用本文复制引用
许耘祥,张莉汶,王朋,顾迎晨,Madan Lal Kolhi,张标,朱莹莹,刘海伟..不同钾水平下烟草根内皮层木栓化的适应性发育差异[J].植物学报,2025,60(6):914-930,17.基金项目
中国烟草总公司山东省公司科技重大专项(No.202417)、山东省自然科学基金(No.ZR2023MC150)和中国农业科学院科技创新工程(No.ASTIP-TRIC03) （No.202417）
