中外医学研究2025，Vol.23Issue(32)：22-26,5.DOI:10.14033/j.cnki.cfmr.2025.32.006
靶向高通量测序对医院获得性肺炎的病原学诊断价值
The Diagnostic Value of Targeted Next-generation Sequencing in Pathogen Detection for Hospital-Acquired Pneumonia
张生宾 1肖俊锐 1刘钊鸿 1黄钟雄 1王杰婷1
作者信息
- 1. 汕头市中医医院 广东 汕头 515041
- 折叠
摘要
Abstract
Objective:To explore the application value of targeted high-throughput sequencing(tNGS)technology for bronchoalveolar lavage fluid(BALF)in the etiological diagnosis of hospital-acquired pneumonia(HAP).Method:A total of 184 patients with HAP admitted to Shantou Hospital of Traditional Chinese Medicine from October 2023 to October 2024 were selected as the research subjects.Based on bronchoalveolar lavage fluid(BALF),the differences in pathogen distribution detected by tNGS and traditional microbial culture were compared,and the differences in diagnostic efficacy of the two methods for mixed infections were further analyzed.Result:The total positive rate of tNGS detection was higher than that of traditional microbial culture(79.35%),but the consistency of the results between the two methods was low,and the difference was statistically significant(P<0.05).The detection rates of tNGS in bacteria and fungi(79.91%,66.85%)were higher than those in traditional microbial cultures(57.07%,32.07%),and the difference was statistically significant(P<0.05).Among 184 patients with HAP,93 were single infections,79 were multiple infections,and 12 had no clear pathogen.Among the mixed infections,the most common infection pattern was bacteria-bacterial infection(45.6%,36/79),followed by bacteria-fungal infection(27.85%,22/79)and bacteria-viral infection(10.1%,8/79).Conclusion:tNGS is superior to conventional microbial culture in the etiological diagnosis of BALF in patients with HAP.It can be used as a supplementary test to traditional microbial culture to provide diagnostic basis for patients with HAP.关键词
靶向高通量测序技术/医院获得性肺炎/支气管肺泡灌洗液/病原微生物/微生物培养Key words
Targeted next-generation sequencing/Hospital-acquired pneumonia/Bronchoalveolar lavage fluid/Pathogenic microorganism/Microbial culture引用本文复制引用
张生宾,肖俊锐,刘钊鸿,黄钟雄,王杰婷..靶向高通量测序对医院获得性肺炎的病原学诊断价值[J].中外医学研究,2025,23(32):22-26,5.
