植物营养与肥料学报2025，Vol.31Issue(11)：2169-2180,12.DOI:10.11674/zwyf.2025144
微藻肥对土壤磷素有效性和团聚体稳定性的影响
Effects of microalgal fertilizer on soil phosphorus availability and soil aggregate stability
摘要
Abstract
[Objectives]This study aimed to investigate the effects of phosphorus fertilizers,particularly microalgal fertilizers,on the composition and stability of soil aggregates and the content of available phosphorus,so as to provide a theoretical basis for selecting new,efficient,and environmentally friendly fertilizers.[Methods]An indoor incubation experiment was conducted using low-phosphorus soil collected from long-term unfertilized plots at the Yucheng Experimental Base of the Dezhou Saline-Alkali Soil Improvement Experimental Station,Chinese Academy of Agricultural Sciences.The experiment treatments composed of a no fertilizer control(CK),two chemical fertilizer treatments[compound fertilizer(CF)and ammonium polyphosphate fertilizer(APP)],and three microalgal fertilizer treatments[wild-type algal fertilizer(AF-WT),high-accumulation polyphosphate algal fertilizer(AF-HP),and low-accumulation phosphate algal fertilizer(AF-LP)].Soil alkaline phosphatase(ALP)activity,aggregate composition(>2 mm,0.25-2 mm,0.053-0.25 mm,and<0.053 mm),aggregate stability indicators(mean weight diameter,MWD;geometric mean diameter,GMD;percentage of water-stable aggregates>0.25 mm,WR>0.25),and available P content(AP)in aggregates were measured.Principal component analysis(PCA)was employed to analyze the dynamic effects of different treatments on soil properties.[Results]Compared with the control(CK),microalgal fertilizer treatments(AF-WT,AF-HP,AF-LP)all increased soil alkaline phosphatase(ALP)activity.The AF-HP treatment exhibited the highest enzyme activity[1.02 nmol/(g·h)]at 90 days of incubation,while the activities of chemical fertilizer treatments CF and APP were 28.80%and 25.58%lower than that of CK,respectively.At 90 days of incubation,AF-HP and AF-WT treatments significantly increased the proportion of aggregates with particle size of 0.053-0.25 mm and remarkably decreased the proportion of aggregates with particle size<0.053 mm.In contrast,CF and APP treatments increased the proportion of aggregates with particle size of 0.25-2 mm in the short term,but the long-term effect diminished.Microalgal fertilizer enhanced the mean weight diameter(MWD)and geometric mean diameter(GMD)of soil aggregates,the AF-HP treatment exhibited a relatively higher MWD value of 0.72,which was 25.10%higher than that of CK at 30 days of incubation.At 90 days of incubation,the available phosphorus contents in aggregates with particle size>2 mm under AF-HP and AF-WT treatments were 2.90 mg/kg and 2.79 mg/kg,respectively,which were 2.15 and 2.07 times higher than that of CK.Chemical fertilizer treatments showed large fluctuations in available phosphorus content,and their long-term effects were weaker than those of microalgal fertilizers.The AF-HP treatment significantly enhanced the contribution rate of available phosphorus in macroaggregates,reaching 53.58%at 90 days of incubation,which was 32.27 percentage points higher than that of CK.The increases in CF and APP treatments were relatively small,with contribution rates of 27.22%and 37.28%,respectively.Principal component analysis(PCA)revealed that AF-HP was positively correlated with available phosphorus and ALP at 90 days of incubation.AF-LP promoted enzyme activity in the short term(3 days),but the correlation weakened in the long term.[Conclusions]AF-HP demonstrates significant advantages in long-term phosphorus supply and enzyme activity activation,while AF-LP excels in short-term enhancement of enzyme activity.APP improves aggregate structure in the short term,whereas CF shows limited comprehensive effects.By increasing alkaline phosphatase activity and promoting the formation of 0.25-2 mm and 0.053-0.25 mm aggregates,microalgal fertilizer enhances the available phosphorus content in soil aggregates of various sizes,significantly improving soil structural stability and phosphorus availability.关键词
微藻肥/土壤团聚体/团聚体稳定性/速效磷Key words
microalgal fertilizer/soil aggregates/aggregate stability/available phosphorus引用本文复制引用
任素娜,杨玉杰,罗冰冰,阮文渊,王龙,易可可..微藻肥对土壤磷素有效性和团聚体稳定性的影响[J].植物营养与肥料学报,2025,31(11):2169-2180,12.基金项目
国家自然科学基金项目(32102478,32472823) （32102478,32472823）
中国博士后科学基金项目(2021M693449,2022T150707) （2021M693449,2022T150707）
北方干旱半干旱耕地高效利用全国重点实验室开放课题(EUAL-2025-07). （EUAL-2025-07）
